In the Literature

TOKU-E Company products are widely distributed to major life science companies and research labs around the world. Over the past several years, scientists in various disciplines from cell culture to chemical engineering have trusted the quality of TOKU-E’s products for use in their invaluable research. TOKU-E Company’s vast customer base and reputation for quality products has led to numerous publications of peer reviewed articles which have referenced TOKU-E products. Below, we have highlighted and summarized some of the interesting research our customers have published and made available online.

Analytical Chemistry

Poller et al. 
used enrofloxacin HCl from TOKU-E to study different methods for detecting enrofloxacin in milk.
Read more here: "Influence of different surface chemistries on the ultrasensitive on-chip detection of enrofloxacin in milk"

Waterman et al. 
used bacitracin F, EvoPure from TOKU-E as a reference standard to confirm the formation of the degradant product, bacitracin F from the active, bacitracin A.
Read more here: "Accelerated Stability Modeling for Peptides: a Case Study with Bacitracin."

Lim et al. 
used polymyxin B1, B2, B3, and B1-I from TOKU-E to study the stability of each compound in saline, dextrose, and saline/dextrose infusion solutions.
Read more here: "Physicochemical stability study of polymyxin B in various infusion solutions for administration to critically Ill patients." 


Huang et al. 
used gentamicin A sulfate, EvoPure®gentamicin X2 sulfate, EvoPure® and kanamycin B sulfate (TOKU-E) to study enzymatic conversion of gentamicin A2 to other gentamicin congeners.
Read more here: "Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex."  

Guo et al. 
used gentamicin X2 sulfate, EvoPure from TOKU-E to study its conversion to G418 by C-methylation at C-6'.
Read more here: "Specificity and Promiscuity at the Branch Point in Gentamicin Biosynthesis.

Mammalian Cell Culture

Lu et al. 
used blasticidin S HCl and puromycin dihydrochloride from TOKU-E to select for transfected AS-B145 and BT-474 cells. 
Read more here: "Ovatodiolide Inhibits Breast Cancer Stem/Progenitor Cells through SMURF2-Mediated Downregulation of Hsp27"

Conti et al. 
puromycin DiHCl from TOKU-E to select for eGFP expressing A549 cells.
Read more here: "Polymeric Nanocarriers And Their Oral Inhalation Forumlations For The Regional Delivery of Nucleic Acids To The Lungs."

Sandoval-Jaime et al. 
used puromycin DiHCl from TOKU-E to select for stably transfected cells.
Read more here: "Recovery of murine norovirus and feline calicivirus from plasmids encoding EMCV IRES in stable cell lines expressing T7 polymerase."


Pryjma, et al. 
from the University of British Columbia used TOKU-E kanamycin sulfate to select for transformed kanamycin resistant Campylobacter jejuniVancomycin HCl from TOKU-E was also used in Mueller Hinton (MH) medium as a selective agent to isolate Campylobacter jejuni.
Read more here:  "FdhTU-Modulated Formate Dehydrogenase Expression and Electron Donor Availability Enhance Recovery of Campylobacter jejuni following Host Cell Infection"

Rose et al. 
used rifampicinclarithromycin, and doxycycline from TOKU-E (all donations) in methacrylate-based copolymer films and studied their effects on biofilm formation.
Read more here: "Prevention of Biofilm Formation by Methacrylate-Based Copolymer Films Loaded With Rifampin, Clarithromycin, Doxycycline Alone or in Combination."

Lahiri et al. 
used cefpodoxime to perform MIC susceptibility tests against an extended-spectrum AmpC (ESAC) B-lactamase producing strain of Enterobacter cloacae.
Read more here: “Activity of avibactam against Enterobacter cloacae producing an extended-spectrum class C b-lactamase enzyme” 

Fox et al. 
studied the synergistic effects of oxacillin sodiumneomycin sulfate (TOKU-E) and blue light irradiation against Staphylococcus aureus.
Read more here: "Investigating Inhibitory Synergy between Blue Light Irradiation and Antibiotic Treatment of Staphylococcus aureus.

Alm et al. 
used cefpodoxime from TOKU-E against Escherichia coli NDM isolates in microdilution MIC assays.
Read more here: "Characterization of Escherichia coli NDM isolates with decreased susceptibility to aztreonam/avibactam: role of a novel insertion in PBP3."

Tian et al. 
used cefsulodin sodium from TOKU-E to study the mechanisms of resistance in cefsulodin-resistant Pseudomonas aeruginosa.
Read more here: "CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa." 

Kaul et al. 
used cefdinir from TOKU-E to study synergistic effects of cefdinir and the prodrug, TXA709 against Methicillin-Resistant Staphylococcus aureus (MRSA).
Read more here: "Combining the FtsZ-Targeting Prodrug TXA709 and the Cephalosporin cefdinir confers synergy and reduces the frequency of resistance in Methicillin-resistant Staphylococcus aureus." 

Sabaeifard et al. 
used amikacin hydrate from TOKU-E to study its cytotoxicity when used in combination with PLGA nanoparticles.
Read more here: "Amikacin loaded PLGA nanoparticles against Pseudomonas aeruginosa."

Kassamali, et al. 
used polymyxin B1polymyxin B2polymyxin B3, and polymyxin B1-I to test for synergistic and antagonistic effects against various Gram-negative organisms.
Read more here: "Microbiological Assessment of Polymyxin B Components Tested Alone and In Combination" 

Molecular Biology

Choi et al. 
used nitrocefin from TOKU-E to study nitrocefin hydrolysis activity of Ni-NTA proteins.
Read more here: "The interplay between effector binding and allostery in an engineered protein switch" 

Lefurgy et al. 
used nitrocefin from TOKU-E to study cephamycin resistance in FOX enzymes which confer resistance to the cephamycin antibiotic, cefoxitin.
Read more here: "FOX-4 cephamycinase: an analysis of structure and function"

Randall et al. 
used nitrocefin from TOKU-E to study PenA B-lactamase activity - a beta-lactamase produced by Burkholderia pseudomallei which confers resistance to ceftazidime.
Read more here: "Membrane bound PenA B-lactamase of Burkholderia pseudomallei"

Kadir et al. 
used cycloheximide from TOKU-E in culture medium to prevent de-novo translation of DVL proteins in neuroblastoma SH-SY5Y cells.
Read more here: "ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size" 

Nicholes et al. 
used nitrocefin from TOKU-E to study the enzymatic activity of protein switches.
Read more here: "Modular protein switches derived from antibody mimetic proteins" 

Caldwell et al. 

used gentamicin C1 sulfate from TOKU-E to study aminoglycoside binding sites of APH-GMPPNP and APH-GTP crystals.
Read more here: "Antibiotic Binding Drives Catalytic Activation of Aminoglycoside Kinase APH(2″)-Ia."  

Pierre, et al. 

used nitrocefin from TOKU-E to measure the enzymatic acivity of various beta-lactamases.
Read more here: "Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein."

Tullman and Nicholes, et al.
used nitrocefin from TOKU-E to study and characterize enzymatic protein switches.
Read more here: "Enzymatic protein switches built from paralogous input domains."

Chen et al. 
used rapamycin from TOKU-E to study the mammalian cellular target of rapamycin.
Read more here: "AMPA receptor–mTOR activation is required for the antidepressant-like effects of sarcosine during the forced swim test in rats: insertion of AMPA receptor may play a role

Eze E et al. 
used nitrocefin from TOKU-E to confirm beta-lactamase production in E. coli and Klebsiella species from Nigeria.
Read more here: "Occurrence of Beta-Lactamases and the Antibiogram Pattern of Clinical Isolates of Escherichia coli and Klebsiella Species in Nsukka Metropolis." 

Choi et al. 
measured enzymatic activity of engineered protein switches by exploiting nitrocefin hydrolysis.
Read more here: "Electrochemical Activation of Engineered Protein Switches."

Kiianitsa et al. 
used nalidixic acid from TOKU-E to study DNA gyrase inhibition in E. coli K-12.
Read more here: "Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay"

Zhangming et al. 
used nitrocefin from TOKU-E as a substrate to study TEM-1 ß-lactamase activity from E. coli.
Read more here: "Label-Free Measurements of Reaction Kinetics Using a Droplet-Based Optofluidic Device."  

Liu et al. 
used nitrocefin from TOKU-E to study and develop a homogeneous biosensor using a non-interfering blocking agent.
Read more here: "Parts-per-Million of Polyethylene Glycol as a Non-Interfering Blocking Agent for Homogeneous Biosensor Development."

Dahlin et al. 
used nitrocefin from TOKU-E in a surrogate ß-lactamase-nitrocefin assay.
Read more here: "A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation."


Ohlhoff et al. 
used nitrocefin from TOKU-E as a substrate to study the activity of EstG34 ß-lactamases.
Read more here: "An unusual feruloyl esterase belonging to family VIII esterases and displaying a broad substrate range"

Huang et al. 
used nitrocefin from TOKU-E as a substrate to study the activity of VIM-2 Metallo-ß-lactamases (MBLs).
Read more here: "Inhibiting the VIM-2 Metallo-ß-Lactamase by Graphene Oxide and Carbon Nanotubes."

Langer et al. 
used bacitracin UltraPure from TOKU-E to inhibit ATG-mediated TF activation and surface-associated PDI activity in HL60 cells.
Read more here: "Rapid Activiation of Monocyte Tissue Factor by Antithymocyte globulin is Dependent on Complement and protein Disulfide Isomerase."

Baradaran-Heravi et al. 
used G418 disulfate from TOKU-E to study premature termination codon readthrough in H1299 cells.
Read more here:"Novel small molecules potentiate premature termination codon readthrough by aminoglycosides."


Webb et al. 
used Bacitracin F, EvoPure to study its effect on protein disulfide isomerase (PDI) inhibition.
Read more here:"Dose-Response Models Reveal Critical Features of Inhibitor Activity and Viral Infection."