• IPTG packaged and labeled.

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SKU: I011

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Description

IPTG is a compound that induces transcription of the lac operon by  mimicking allolactose, a lactose metabolite. IPTG is routinely used as a tool in cloning and gene recombination to confirm successful gene insertion. 

    CAS Number

    367-93-1

    Molecular Formula

    C9H18O5S

    Molecular Weight

    238.3

    Mechanism of Action

    IPTG induces the lac operon by binding to and releasing the lac repressor allowing transcription of lac genes including beta-galactosidase.

    Storage Conditions

    2-8┬░C

    Tariff Code

    2932.99.9090

Applications

    Microbiology Applications

    IPTG can be used to confirm successful DNA recombination within the lac operon in cloning experiments. X-gal, a chromogenic substrate which turns a blue color when cleaved by IPTG can be used in the media in which the cells are grown. White colonies indicate a gene insertion within the lac operon because beta-galactosidase was not expressed. Blue colonies are indicative of an intact lac operon which expressed beta-galactosidase and subsequently cleaved X-gal.

Specifications

    Form

    Powder

References

    References

    Chang, Lewis M., and N. C. Horton. "Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer." Europe PubMed Central 271.5253 (1996): 1247-254. Europepmc.org. Web. 4 Dec. 2012.

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