Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

Page 2 of 5 1 2 3 4 5
Methotrexate is a selective agent for DHFR transfected cells and an anticancer agent.
Nourseothricin (Streptothricin) Sulfate
Nourseothricin is an antibiotic in the Streptothricin class of antibiotics.
Puromycin aminonucleoside packaged and labeled.

Puromycin Aminonucleoside is the aminonucleoside portion of Puromycin, used in animal models of nephrosis.

Spectinomycin dihydrochloride pentahydrate packaged and labeled.
Spectinomycin dihydrochloride pentahydrate is an aminocyclitol antibiotic that is isolated from Streptomyces spectabilis.  Spectinomycin was...
Spectinomycin sulfate packaged and labeled.
Spectinomycin Sulfate is an aminocyclitol antibiotic that is isolated from Streptomyces spectabilis.  Spectinomycin was discovered in...
Tetracycline hydrochloride packaged and labeled.
Tetracycline HCl is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by...
Ampicillin trihydrate, USP packaged and labeled.

Ampicillin Trihydrate is β-lactam antibiotic commonly used as a selectable marker.

Bleomycin A5 hydrochloride
Bleomycin A5 hydrochloride is a Bleomycin complex with anti-cancer properties.
Carbenicillin disodium, USP packaged and labeled.
Carbenicillin disodium, USP is a carboxypenicillin antibiotic routinely used in gene seleciton.
Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is the major product of chloramphenicol acetyltransferase.
Erythromycin Ethylsuccinate packaged and labeled.
Erythromycin Ethylsuccinate, an ethylsuccinate salt form of Erythromycin, is a broad-spectrum macrolide antibiotic.
G418 Disulfate (Low Endotoxin)
G418 Disulfate (Low Endotoxin) is a form of G418 Disulfate with low endotoxin content  (≤1 EU/mg).
Page 2 of 5 1 2 3 4 5