Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
Page 1 of 3 1 2 3
Packaged and labeled G418 disulfate.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
Hygromycin B packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely...
Blasticidin S HCl packaged and labeled in glass bottle.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes and is routinely used in..
Actinomycin D packaged and labeled.
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis...
Blasticidin S packaged and labeled in glass vial.
Blasticidin S is a nucleoside produced by several species of Streptomyces, first reported...
G418 disulfate, EvoPure packaged and labeled.
G418 disulfate, EvoPure® is a high purity (≥99.0%) form of G418 disulfate and is free of impurities present in standard grade G418...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® solution (100 mg/mL) is a solution containing high purity (>99.0%) hygromycin B...
Methotrexate
Methotrexate is a selective agent for DHFR transfected cells and an anticancer...
Methotrexate, EvoPure<sup>&reg;</sup>

Methotrexate is a selective agent for DHFR transfected cells and an anticancer compound that inhibits the metabolism...

Mitomycin C packaged and labeled.
Mitomycin C is a methylazirinopyrroloindoledione antineoplastic antibiotic isolated Streptomyces spp.
Nourseothricin (streptothricin) sulfate
Nourseothricin is an antibiotic in the Streptothricin class of antibiotics.
Puromycin aminonucleoside packaged and labeled.
Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is...
Page 1 of 3 1 2 3