Tetracycline hydrochloride packaged and labeled.

T00425 g
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  • Description

    Tetracycline is a bacteriostatic polyketide antibiotic frequently used in a wide range of in vitro cell culture applications.  TOKU-E offers three forms of tetrcycline: tetracycline HCL (T004), tetracycline, USP (T051), and tetracycline, EP (T016).  Tetracycline, USP and tetracycline, EP are sparingly soluble in aqueous solution at 0.231 mg/mL.  Tetracycline HCl, is slighly soluble in aqueous solution at 10mg/mL.

    Mechanism of Action

    Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA from binding to the “A” site in the bacterial ribosome.


    Tetracycline HCl is a broad spectrum antibiotic targeting a wide range of gram positive and gram negative bacteria responsible for upper respiratory tract infections.

    • Microbiology Applications

      Tetracycline HCl is routinely used as a selective agent to select for bacterial cells that have been transformed with a plasmid that contains the tetracycline resistance gene, tet. Tetracycline is typically used at 10 µg/mL.

    • Plant Biology Applications

      Tetracycline has shown to suppress aster yellows disease symptoms on China Aster and Chrysanthemum plants. Multiple applications of tetracycline resulted in symptomless axillary plant growth (Davis R.E. and Whitcomb, 1970).

    • Eukaryotic Cell Culture Applications

      Tetracycline is routinely used to select for cells containing resistance plasmids in cell lines such as HeLa at an effective concentration of 1µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    • References

      Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

      Davis R.E. and Whitcomb, 1970, R.F. Evidence on Possible Mycoplasma Etiology of Aster Yellows Disease. Infection and Immunity, Aug. 1970, p. 201-208



    Tariff Code


    Related Documents

    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to [email protected]

  • CAS Number64-75-5
    Molecular FormulaC22H24N2O8 •HCl
    Molecular Weight480.90
    AppearanceYellow crystalline powder
    Impurity Profile4-Epitetracycline: Not more than 3.0%
    Anhydrotetracycline: Not more than 0.5%
    4-Epianhydrotetracycline: Not more than 0.5%
    2-Acetyl-2-decarbamoyl-tetracycline: Not more than 1.5%
    Heavy Metals: ≤50ppm
    Sulphate Ash: ≤0.5%
    Bacterial Endotoxin: ≤0.5 IU/mg
    SourceStreptomyces sp.
    Potency (on a dry basis)≥900µg/mg
    Optical Rotation-240° to -255°
    Storage Conditions-20°C
    Assay(On Dried Basis): 95.0-102.0%
    Loss on Drying≤2.0%
    Residual Solvents1-Butanol: Not more than 5000ppm
    Acetone: Not more than 5000ppm
  • Documents