Cefsulodin sodium is a third generation cephalosporin antibiotic.
Recently, TOKU-E has found that the main cause of cefsulodin instability stems from one key impurity in 7-ACA (7-aminocephalosporanic acid- a raw material used in the synthesis of cefsulodin). In order to produce high-purity, high-stability cefsulodin, TOKU-E uses industrial HPLC to remove significant quantities of this impurity in 7-ACA and thus produce ultra-pure, ultra-stable, and ultra-potent cefsulodin.
Tian et al. used cefsulodin sodium from TOKU-E to study the mechanisms of resistance in cefsulodin-resistant Pseudomonas aeruginosa. Read more here: "CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa."
Mechanism of Action
Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.
Cefsulodin sodium has a very limited spectrum specifically targeting Pseudomonas aeruginosa. Other members of the gram positive and gram negative species show little susceptibility.
Cefsulodin sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) primarily against Pseudomonas aeruginosa isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
- Pseudomonas aeruginosa 128 µg/mL – 512 µg/mL
- For a complete list of cefsulodin MIC values, click here.
Cefsulodin can be used as a selective agent in several types of isolation media:
Yersinia Selective Agar - Yersinia selective supplement
Columbia Blood Agar - Helicobacter pylori Selective Supplement (Dent)
mTSB - VCC Selective Supplement
Salmonella Chromogenic Agar - Salmonella Selective Supplement
Georgopapadakou, N. H. "Mechanisms of Action of Cephalosporin 3'-quinolone Esters, Carbamates, and Tertiary Amines in Escherichia Coli." American Society for Microbiology 37.3 (1992): 559-65. Antimicrobial Agents and Chemotherapy. Web. 21 Aug. 2012.
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Cefsulodin Stability Study
Cefsulodin 0.5mg/mL solution was observed to not degrade over a 4 day span at room temperature. With light additional heating (35°C, 1 hour) the cefsulodin powder didn’t degrade while the solution showed slight degradation.
Additional heating (35°C, 5 hours) of the solution yielded 6% of degradation of Cefsulodin solution.
Stability at room temperature for >7 days fell below the >90% purity threshold as well as amount of cefsulodin.