Amphotericin B, EP packaged and labeled.

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  • Description

    Amphotericin B, EP is a polyene antifungal or antimycotic compound derived from Streptomyces nodosus. Amphotericin B, EP is nearly insoluble in water but can be solubilized in sodium deoxycholate.

    Amphotericin B, EP meets European Pharmacopoeia specifications.

    Mechanism of Action

    Amphotericin B associates with membrane sterols in mammalian and fungal cell membranes. Amphotericin B forms a pore in these membranes leading to essential ion leakage and ultimately, cell death.

    Spectrum

    Amphotericin B is active against mammalian and fungal (molds and yeasts) cells. Amphotericin B is not toxic to bacteria due to their lack of sterols.

    The following represents MIC susceptibility data for amphotericin B against common fungal pathogens:

    • Candida albicans - 0.001 - 321 μg/mL
    • Candida krusei - 0.001 - 16 μg/mL
    • Coccidioides immitis - 0.0625 - 2 μg/mL
    • Cryptococcus neoformans - 0.2 - 39 μg/mL
    • Fusarium oxysporum - 0.75 - 125 μg/mL

    • Microbiology Applications

      Amphotericin B is used as an antimycotic selective agent in several routinely used selective media formulations to inhibit the growth of background fungal growth.

    • Eukaryotic Cell Culture Applications

      Amphotericin B can be used in eukaryotic cell culture to control or prevent fungal contamination. Amphotericin B can be toxic to cell lines at high concentrations and should not be used at concentrations greater than 2.50 µg/mL.

    • References

      Rice, Louis B., and Mahmoud A. Ghannoum. "Antifungal Agents: Mode of Action, Mechanisms of Resistance, and Correlation of These Mechanisms with Bacterial Resistance." Clinical Microbiology Reviews (1999): 501-17. www.ncbi.gov. Oct. 1999. Web. 22 Aug. 2012.

      Rice L.B. and Ghannoum M.A.. "Antifungal Agents: Mode of Action, Mechanisms of Resistance, and Correlation of These Mechanisms with Bacterial Resistance. Clinical Microbiology Reviews (1999): 501-17.

      Brajtburg, J., W. G. Powderly, and G. Medoff. "Amphotericin B: Current Understanding of Mechanisms of Action." Antimicrobial Agents and Chemotherapy 34.2 (1990): 183-88. www.ncbi.gov. Web. 22 Aug. 2012.

      Perez-de-Luque A., Cifuentes Z., Beckstead J.A., Sillero J.C., Avila C., Rubio J. and Ryan R.O.. Effect of amphotericin B nanodisks on plant fungal diseases. Volume 68, Issue 1, Article first published online: 24 Jun 2011.

    Form

    Powder

    Tariff Code

    2941.90.1050

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    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to info@toku-e.com

  • CAS Number1397-89-3
    Molecular FormulaC47H73NO17
    Molecular Weight924.08
    FormPowder
    AppearanceYellow or Orange hygroscopic powder
    SolubilityInsoluble: H2O, Ethanol
    Soluble: Dimethyl sulfoxide, propylene glycol, slightly soluble in methanol
    SourceStreptomyces nodosus
    AbsorbanceUV max: 362, 381 and 405nm
    UV ratios:
    A362/A381 = 0.57-0.61
    A381/A405 = 0.87-0.93
    Storage Conditions2-8°C
    Assay(On Dried Basis): ≥750 IU/mg
    Loss on Drying≤5.0%
    Sulfated Ash≤3.0%
    ImpuritiesRelated Substances
    Impurity A (303 nm): ≤5.0%
    Any Other Impurity (303 nm): ≤1.0%
    Impurity B (383 nm): ≤4.0%
    Any Other Impurity (383 nm): ≤2.0%
    Total Impurities (303 nm and 383 nm): ≤15.0%
    IdentificationUV, IR, HPLC, and by an EP designed experiment