Amphotericin B, EP is a polyene antifungal or antimycotic compound derived from Streptomyces nodosus. Amphotericin B, EP is nearly insoluble in water but can be solubilized in sodium deoxycholate.
Amphotericin B, EP meets European Pharmacopoeia specifications.
Mechanism of Action
Amphotericin B associates with membrane sterols in mammalian and fungal cell membranes. Amphotericin B forms a pore in these membranes leading to essential ion leakage and ultimately, cell death.
Amphotericin B is active against mammalian and fungal (molds and yeasts) cells. Amphotericin B is not toxic to bacteria due to their lack of sterols.
The following represents MIC susceptibility data for amphotericin B against common fungal pathogens:
- Candida albicans - 0.001 - 321 μg/mL
- Candida krusei - 0.001 - 16 μg/mL
- Coccidioides immitis - 0.0625 - 2 μg/mL
- Cryptococcus neoformans - 0.2 - 39 μg/mL
- Fusarium oxysporum - 0.75 - 125 μg/mL
Amphotericin B is used as an antimycotic selective agent in several routinely used selective media formulations to inhibit the growth of background fungal growth.
Eukaryotic Cell Culture Applications
Amphotericin B can be used in eukaryotic cell culture to control or prevent fungal contamination. Amphotericin B can be toxic to cell lines at high concentrations and should not be used at concentrations greater than 2.50 µg/mL.
Rice, Louis B., and Mahmoud A. Ghannoum. "Antifungal Agents: Mode of Action, Mechanisms of Resistance, and Correlation of These Mechanisms with Bacterial Resistance." Clinical Microbiology Reviews (1999): 501-17. www.ncbi.gov. Oct. 1999. Web. 22 Aug. 2012.
Rice L.B. and Ghannoum M.A.. "Antifungal Agents: Mode of Action, Mechanisms of Resistance, and Correlation of These Mechanisms with Bacterial Resistance. Clinical Microbiology Reviews (1999): 501-17.
Brajtburg, J., W. G. Powderly, and G. Medoff. "Amphotericin B: Current Understanding of Mechanisms of Action." Antimicrobial Agents and Chemotherapy 34.2 (1990): 183-88. www.ncbi.gov. Web. 22 Aug. 2012.
Perez-de-Luque A., Cifuentes Z., Beckstead J.A., Sillero J.C., Avila C., Rubio J. and Ryan R.O.. Effect of amphotericin B nanodisks on plant fungal diseases. Volume 68, Issue 1, Article first published online: 24 Jun 2011.
For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to [email protected]