• Tetracycline hydrochloride packaged and labeled.

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SKU: T004

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Tetracycline is a bacteriostatic polyketide antibiotic frequently used in a wide range of in vitro cell culture applications.  TOKU-E offers three forms of tetrcycline: tetracycline HCL (T004), tetracycline, USP (T051), and tetracycline, EP (T016).  Tetracycline, USP and tetracycline, EP are sparingly soluble in aqueous solution at 0.231 mg/mL.  Tetracycline HCl, is slighly soluble in aqueous solution at 10mg/mL.

    CAS Number


    Molecular Formula

    C22H24N2O8 •HCl

    Molecular Weight


    Mechanism of Action

    Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA from binding to the “A” site in the bacterial ribosome.

    Storage Conditions


    Tariff Code



    Tetracycline HCl is a broad spectrum antibiotic targeting a wide range of gram positive and gram negative bacteria responsible for upper respiratory tract infections.


    Eukaryotic Cell Culture Applications

    Tetracycline is routinely used to select for cells containing resistance plasmids in cell lines such as HeLa at an effective concentration of 1µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Tetracycline HCl is routinely used as a selective agent to select for bacterial cells that have been transformed with a plasmid that contains the tetracycline resistance gene, tet. Tetracycline is typically used at 10 µg/mL.

    Plant Biology Applications

    Tetracycline has shown to suppress aster yellows disease symptoms on China Aster and Chrysanthemum plants. Multiple applications of tetracycline resulted in symptomless axillary plant growth (Davis R.E. and Whitcomb, 1970).





    Yellow crystalline powder


    Streptomyces sp.

    Impurity Profile

    4-Epitetracycline: Not more than 3.0%
    Anhydrotetracycline: Not more than 0.5%
    4-Epianhydrotetracycline: Not more than 0.5%
    2-Acetyl-2-decarbamoyl-tetracycline: Not more than 1.5%
    Heavy Metals: ≤50ppm
    Sulphate Ash: ≤0.5%
    Bacterial Endotoxin: ≤0.5 IU/mg

    Potency (on a dry basis)




    Optical Rotation

    -240° to -255°


    (On Dried Basis): 95.0-102.0%

    Loss on Drying


    Residual Solvents

    1-Butanol: Not more than 5000ppm
    Acetone: Not more than 5000ppm



    Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

    Davis R.E. and Whitcomb, 1970, R.F. Evidence on Possible Mycoplasma Etiology of Aster Yellows Disease. Infection and Immunity, Aug. 1970, p. 201-208

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