• Tetracycline hydrochloride packaged and labeled.

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SKU: T004

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Description

Tetracycline is a bacteriostatic polyketide antibiotic frequently used in a wide range of in vitro cell culture applications.  TOKU-E offers three forms of tetrcycline: tetracycline HCL (T004), tetracycline, USP (T051), and tetracycline, EP (T016).  Tetracycline, USP and tetracycline, EP are sparingly soluble in aqueous solution at 0.231 mg/mL.  Tetracycline HCl, is slighly soluble in aqueous solution at 10mg/mL.

    CAS Number

    64-75-5

    Molecular Formula

    C22H24N2O8 •HCl

    Molecular Weight

    480.90

    Mechanism of Action

    Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA from binding to the “A” site in the bacterial ribosome.

    Storage Conditions

    -20°C

    Tariff Code

    2941.30.0000

    Spectrum

    Tetracycline HCl is a broad spectrum antibiotic targeting a wide range of gram positive and gram negative bacteria responsible for upper respiratory tract infections.

Applications

    Eukaryotic Cell Culture Applications

    Tetracycline is routinely used to select for cells containing resistance plasmids in cell lines such as HeLa at an effective concentration of 1µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Tetracycline HCl is routinely used as a selective agent to select for bacterial cells that have been transformed with a plasmid that contains the tetracycline resistance gene, tet. Tetracycline is typically used at 10 µg/mL.

    Plant Biology Applications

    Tetracycline has shown to suppress aster yellows disease symptoms on China Aster and Chrysanthemum plants. Multiple applications of tetracycline resulted in symptomless axillary plant growth (Davis R.E. and Whitcomb, 1970).

Specifications

    Form

    Powder

    Appearance

    Yellow crystalline powder

    Source

    Streptomyces sp.

    Impurity Profile

    4-Epitetracycline: Not more than 3.0%
    Anhydrotetracycline: Not more than 0.5%
    4-Epianhydrotetracycline: Not more than 0.5%
    2-Acetyl-2-decarbamoyl-tetracycline: Not more than 1.5%
    Heavy Metals: ≤50ppm
    Sulphate Ash: ≤0.5%
    Bacterial Endotoxin: ≤0.5 IU/mg

    Potency (on a dry basis)

    ≥900µg/mg

    pH

    1.8-2.8

    Optical Rotation

    -240° to -255°

    Assay

    (On Dried Basis): 95.0-102.0%

    Loss on Drying

    ≤2.0%

    Residual Solvents

    1-Butanol: Not more than 5000ppm
    Acetone: Not more than 5000ppm

References

    References

    Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

    Davis R.E. and Whitcomb, 1970, R.F. Evidence on Possible Mycoplasma Etiology of Aster Yellows Disease. Infection and Immunity, Aug. 1970, p. 201-208

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