• Rifampicin packaged and labeled.

click on thumbnail to zoom
SKU: R003

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Rifampicin is a sparingly soluble (1.4 mg/mL) bacterial protein synthesis inhibiting antibiotic.

For more rifampicin products, click here.

    CAS Number

    13292-46-1

    Molecular Formula

    C43H58N4O12

    Molecular Weight

    822.94

    Mechanism of Action

    Rifampicin targets prokaryotic DNA dependent RNA polymerases which prevent subsequent RNA transcription and protein translation.

    Storage Conditions

    Protect from light at -20°C.

    Tariff Code

    2941.90.5000

    Spectrum

    Rifampicin is a broad spectrum antibiotic targeting a wide range of gram positive, gram negative, and Mycobacterium species.

Applications

    Microbiology Applications

    Rifampicin is commonly used in bacterial recombinant protein expression to inhibit bacterial RNA polymerase activity and synthesis of host bacterial proteins. Rifampicin can also be used as a selective agent to isolate Campylobacter jejuni.

    Rose et al. used rifampicin from TOKU-E in methacrylate-based copolymer films and studied its effects on biofilm formation: "Prevention of Biofilm Formation by Methacrylate-Based Copolymer Films Loaded With Rifampin, Clarithromycin, Doxycycline Aone or in Combination." 

    Media Supplements

    Rifampicin can be used as a selective agent in several types of isolation media:

    Campylobacter Agar - Campylobacter Selective Supplement (Preston)

    Campylobacter Agar Base - Modified Preston Campylobacter Selective Supplement

    Plant Biology Applications

    Rifampicin has been tested in Jerusalem artichoke tuber explants by adding 10 to 50 µg/ml to the tissue culture medium. At 50 µg/ml no bacterial infection was detectable, without affecting cell division rates, cytodifferentiation and DNA synthesis. As a result Rifampicin was used as antibacterial in the following experiments of this university department (Philips, 1981).

Specifications

    Form

    Powder

    Appearance

    Brownish-red crystalline powder

    Source

    Semi-synthetic: Amycolatopsis Rifamycinica

    Impurity Profile

    Related Substances:
    Rifampicin Quinone: ≤ 1.5%
    Single Peak: ≤ 1.0%
    Sum of Peaks: ≤ 3.5%

    pH

    4.5-6.5

    Density

    ≥ 0.7g/cc

    Assay

    (On Dried Basis): 97.0-102.0%

    Loss on Drying

    ≤1.0%

    Residue On Ignition

    ≤ 0.1%

    Heavy Metals

    ≤20 ppm

References

    References

    "Rifampin: Mechanisms of Action and Resistance." Oxford Journals (1983): n. pag. Clinical Infectious Diseases. Web. 21 Aug. 2012.

    "Philips R., Arnott S.M. and K aplan S.E., 1981, Antibiotics in plant tissue culture: rifampicin effectively controls bacterial contaminants without affecting the growth of short-term explant cultures of Helianthus tuberosus. Plant Science Letters, 21 (1981) 235-240.

Other Items In This Category