• Polymyxin B sulfate packaged and labeled.

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SKU: P007

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Description

Polymyxin B sulfate is a polypeptide antibiotic and is composed of polymyxins B1, B2, and B3 with fractions B1 and B2 comprising the majority of the mixture. Polymyxin B sulfate is freely soluble in aqueous solution (25 mg/mL). Polymyxin B components are structurally identical with the exception of a variable fatty acid group on each fraction. Results from in vitro studies have shown marginal differences in MIC data when comparing the fractions.

TOKU-E offers six forms of polymyxin B sulfate: polymyxin B sulfate (P007), polymyxin B1 sulfate, EvoPure® (P037), polymyxin B1-I sulfate, EvoPure® (P038), polymyxin B2 sulfate, EvoPure® (P039)polymyxin B3 sulfate, EvoPure® (P040), and polymyxin B6 sulfate, EvoPure®. EvoPure® products are purified single antibiotic fractions, most >99% pure. High purity EvoPure® polymyxin products can be used to analyze the specifc effects of individual polymyxin B fractions.

    CAS Number

    1405-20-5

    Molecular Formula

    Mixture

    Molecular Weight

    1385.61

    Mechanism of Action

    Polymyxin B targets and alters the permeability of lipopolysaccharide (LPS) found in Gram negative bacteria leading to lysing of the cell. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.1050

    Spectrum

    Polymyxin B sulfate targets the outer membrane of gram negative bacteria especially Pseudomonas aeruginosa.

Applications

    Microbiology Applications

    Polymyxin B sulfate is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Pseudomonas aeruginosa 0.25 µg/mL – 1 µg/mL
    • For a complete list of polymyxin B sulfate MIC values, click here.

    Media Supplements

    Polymyxin B is routinely used as a selection agent in several types of isolation media:

    Perfringens agar - Supplement A and Supplement B

    Perfringens Agar - SFP and TSC selective supplements

    Columbia Blood AgarCampylobacter selective supplement (Skirrow)

    Columbia Blood Agar - Campylobacter selective supplement (Blaser-Wang)

    Brucella mediumBrucella selective supplement

    MYP Agar - Polymyxin B Bacillus selection supplement

    Legionella CYE Agar - Legionella BMPA-α Selective Supplement

    Legionella CYE Agar - Legionella MWY Selective Supplement

    Campylobacter Agar - Campylobacter Selective Supplement (Preston)

    PALCAM Agar - PALCAM Selective Supplement

    Legionella CYE Agar - Legionella GVPC Selective Supplement

    m-CP Medium - Membrane C. perfringens Selective Supplement

    Burkholderia cepacia Agar Base - Burkholderia cepacia Selective Supplement

    ORSAB - ORSAB Selective Supplement

    Campylobacter Agar Base - Modified Preston Campylobacter Selective Supplement

    Brucella Medium Base - Modified Brucella Selective Supplement

    Legionella CYE Agar - Legionella GVPN Selective Supplement

    ChromogenicListeria Agar - Chromogenic Listeria Selective Supplement

    ChromogenicBacillus cereus Agar - Chromogenic Bacillus cereus Selective Supplement

    ChromogenicListeria Agar - Chromogenic Listeria Differential Supplement

    Plant Biology Applications

    Polymyxin B sulfate was successfully tested to counteract phytopathogenic gram-negative bacterial growth including different strains of Pseudomonas viridiflava and Erwinia carotovora. Polymixin B sulfate was shown to reduce bacterial growth of different strains of Pseudomonas viridiflava at low concentrations, (0.08 µg/ml) and Erwinia carotovora growth at slightly higher concentrations (0.25 µg/ml) (Selim et al. 2005). Polymyxin B has been shown to elict alkaloid accumulation in E. californica. Treatment at 0.04 mg/ml for 4 hours showed a 5.5 times increase in Jasmonate levels over the control (water).

Specifications

    Form

    Powder

    Appearance

    White powder

    Source

    Bacillus Polymyxa

    Impurity Profile

    Polymyxin B1||4135-11-9|C56H98N16O13|1204| Polymyxin B2|||C55H96N16O13|1190| Polymyxin B3|||C55H96N16O13|1190| Polymyxin B1-I|||C56H98N16O13|1204|

    Melting Point

    217-220°C

    Assay

    (Dried Basis): ≥6,000 u/mg

    Loss on Drying

    ≤7.0%

References

    References

    Newton, B. A. "The Properties and Mode of Action of the Polymyxins." Bacteriology Reviews (n.d.): 14-27. www.ncbi.gov. Web. 21 Aug. 2012.

    Selim S., Negrel J., Govaerts C., Gianinazzi S. and Tuinen van D., 2005, Isolation and Partial Characterization of Antagonistic Peptides Produced by Paenibacillus sp. Strain B2 Isolated from the Sorghum Mycorrhizosphere. Applied and Environmental Microbiology, Nov. 2005, p. 6501–6507

    Zavascki, Alexandre Prehn et al. "Polymyxin B for the Treatment of Multidrug-resistant Pathogens: A Critical Review." Journal of Antimicrobial Chemotherapy 60 (2007): 1206-215.Oxfordjournals. Web. 15 Jan. 2013.

    Li, Jian et al. "Development and Validation of a Reversed-phase High-performance Liquid Chromatography Assay for Polymyxin B in Human Plasma." Journal of Antimicrobial Chemotherapy (2009): n. pag. Oxfordjournals. Web. 15 Jan. 2013.

    Tam, Vincent H, et al. "In Vitro Potency of Various Polymyxin B Components." In Vitro Potency of Various Polymyxin B Components 55.9 (2011): 4490-491. Asm.org. Web. 15 Jan. 2013.

    Orwa, J. A., et al "Isolation and Structural Characterization of Polymyxin B Components." Isolation and Structural Characterization of Polymyxin B Components 912.2 (2001): 369-73. Sciencedirect. Web. 15 Jan. 2013.

    MJ Mueller, W Brodschelm. "Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid". Proc. Natl. Acad. Sci. USA Vol. 90, pp. 7490-7494, August 1993.

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