• Polymyxin B1-I sulfate, EvoPure packaged and labeled.

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SKU: P038

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Description

Polymyxin B1-I sulfate is one of the individual polypeptide components in polymyxin B sulfate. Polymyxin B1-I contains, among other amino acids, an L-isoleucine residue in place of the L-leucine reside found in the other polymyxin fractions. The unique fatty acid group found in B1-I is 6-methyloctanoic acid (6-MOA), the same group found in polymyxin B1. Results from in vitro studies have shown marginal differences in MIC data when comparing the fractions.

Kassamali, et al. used polymyxin B1polymyxin B2polymyxin B3, and polymyxin B1-I to test for synergistic and antagonistic effects against various Gram-negative organisms.
Read more here: "Microbiological Assessment of Polymyxin B Components Tested Alone and In Combination" 

Lim et al. used polymyxin B1, B2, B3, and B1-I from TOKU-E to study the stability of each compound in saline, dextrose, and saline/dextrose infusion solutions. "Physicochemical stability study of polymyxin B in various infusion solutions for administration to critically Ill patients."  

    CAS Number

    80469-10-9

    Molecular Formula

    C56H98N16O13  xH2O4S (lot specific)

    Molecular Weight

    1203.47 g/mol (Free base)

    Mechanism of Action

    Polymyxin B targets and alters permeability lipopolysaccharide (LPS) of gram negative bacteria leading to lysing of the cell. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1050

    Spectrum

    Polymyxin B sulfate targets the outer membrane of gram negative bacteria especially Pseudomonas aeruginosa.

    Kassamali, et al. found polymyxin B3 to be the most active polymyxin fraction against most organisms in their experiment. Kassamali, et al. also discovered a synergistic effect using polymyxin B3 and B1-I against their tested organisms.

Applications

    Microbiology Applications

    Polymyxin B sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Pseudomonas aeruginosa 0.25 µg/mL – 1 µg/mL
    • For a complete list of polymyxin B sulfate MIC values, click here.

    Plant Biology Applications

    Polymyxin B sulfate was successfully tested to counteract phytopathogenic gram-negative bacterial growth including different strains of Pseudomonas viridiflava and Erwinia carotovora. Polymixin B sulfate was shown to reduce bacterial growth of different strains of Pseudomonas viridiflava at low concentrations, (0.08 µg/ml) and Erwinia carotovora growth at slightly higher concentrations (0.25 µg/ml) (Selim et al. 2005).

Specifications

    Appearance

    White powder

    Source

    Pseudomonas Sp.

Technical Data

HPLC Chromatogram Showing Ultra High, Single Fraction Purity of Polymyxin B1-I Sulfate, EvoPure®

Polymyxin B1-I Sulfate, EvoPure

References

    References

    Newton, B. A. "The Properties and Mode of Action of the Polymyxins." Bacteriology Reviews (n.d.): 14-27. www.ncbi.gov. Web. 21 Aug. 2012.

    Selim S., Negrel J., Govaerts C., Gianinazzi S. and Tuinen van D., 2005, Isolation and Partial Characterization of Antagonistic Peptides Produced by Paenibacillus sp. Strain B2 Isolated from the Sorghum Mycorrhizosphere. Applied and Environmental Microbiology, Nov. 2005, p. 6501–6507

    Zavascki, Alexandre Prehn et al. "Polymyxin B for the Treatment of Multidrug-resistant Pathogens: A Critical Review." Journal of Antimicrobial Chemotherapy 60 (2007): 1206-215.Oxfordjournals. Web. 15 Jan. 2013.

    Li, Jian et al. "Development and Validation of a Reversed-phase High-performance Liquid Chromatography Assay for Polymyxin B in Human Plasma." Journal of Antimicrobial Chemotherapy (2009): n. pag. Oxfordjournals. Web. 15 Jan. 2013.

    Tam, Vincent H, et al. "In Vitro Potency of Various Polymyxin B Components." In Vitro Potency of Various Polymyxin B Components 55.9 (2011): 4490-491. Asm.org. Web. 15 Jan. 2013.

    Orwa, J. A., et al "Isolation and Structural Characterization of Polymyxin B Components." Isolation and Structural Characterization of Polymyxin B Components 912.2 (2001): 369-73. Sciencedirect. Web. 15 Jan. 2013.

    MJ Mueller, W Brodschelm. "Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid". Proc. Natl. Acad. Sci. USA Vol. 90, pp. 7490-7494, August 1993.

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