• Neomycin sulfate, USP packaged and labeled.

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SKU: N003

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Description

Neomycin Sulfate, USP is a broad-spectrum aminoglycoside antibiotic composed of related compounds including Neomycin A (neamine), Neomycin B (framycetin), Neomycin C, and a few minor compounds.  Neomycin B is the most active, followed by C and A. The quantities of these components vary from lot-to-lot.

Neomycin Sulfate, USP conforms to United States Pharmacopoeia specifications.

For additional Neomycin products, click here.

Custom manufacturing and testing: We are able to prepare custom Neomycin Sulfate suitable for your unique specifications for use in cell culture, or as ancillary material in upstream biopharma manufacturing.  Additionally, we offer additional testing including endotoxin content, arsenic content, cell line testing, spectral analysis, and more. For more information, please contact us.

    CAS Number

    1405-10-3

    Molecular Formula

    C23H46N6O13 · 3 H2SO4

    Molecular Weight

    908.88

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.1010

    Spectrum

    Neomycin is broad-spectrum, but is mostly used against Gram-negative bacteria.

Applications

    Eukaryotic Cell Culture Applications

    Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in cell lines including AtT-20. For additional information, please visit our cell-culture database.

    Microbiology Applications

    Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).

    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:

      • Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
      • Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
      • For a representative list of Neomycin MIC values, click here.

    Neomycin Sulfate can also be used for food testing in TSN agar to select for Clostridium perfringins and inhibit growth of Enterobacteria and Clostridium bifermentans.  

    Plant Biology Applications

    Neomycin is commonly used in gene selection wtih Agrobacterium transformation protocols to select for plants that have taken up the  plasmid conferring resistance to Neomycin.

Specifications

    Form

    Powder

    Appearance

    White or yellowish powder

    Source

    Streptomyces fradiae.

    pH

    5.0-7.5

    Assay

    (Dry basis): ≤ 600µg/mg

    Loss on Drying

    ≤ 8.0%

References

    References

    TOKU-E Reference

    Our Neomycin Sulfate was used to study its synergistic effects with blue light irradiation against S. aureus as a biofilm disruptor in antimicrobial photodynamic therapy in: Investigating inhibitory synergy between blue light irradiation and antibiotic treatment of Staphylococcus aureus (Fox et al, 2013).

    References

    Aragão FJL and Brasileiro ACM (2002) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-10

    Dai et al (2001)  Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol. Breeding 7: 25–33:2001

    Davis BD (1987)  Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev.  51(3):341-50

    Robertson JH (1971)  Antimicrobial activity of Neomycin C against Staphylococcus epidermidis. App. Micro. 22(6):1164-1165

    Tsuji K and Robertson JH (1969)  Comparative study of responses to Neomycins B and C by microbiological and gas-liquid chromatographic assay methods. App. Microbiol.  18(3):396-398

    Yuan L and Wei H (2006) Rapid analysis of native Neomycin components on a portable capillary electrophoresis system with potential gradient detection. Analytic.  Bioanalyt. Chem. 385(8):1575-1579

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