• Methotrexate sodium salt packaged and labeled.

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SKU: M031

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Methotrexate sodium salt is a selective agent for dihydrofolate reductase (DHFR)- transfected cells, and a nucleic acid synthesis inhibitor in protein expression systems, thus can be used as an ancillary material in upstream biomanufacturing applications.     It also has immunosuppressive effects for rheumatoid arthritis research and has anti-cancer properties. Methotrexate sodium salt is freely soluble in aqueous solution.

We also offer:

  • Methotraxate (M027)
  • Methotrexate, EvoPure® (M091)

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for questions.

    CAS Number


    Molecular Formula


    Molecular Weight


    Mechanism of Action

    In cancerous cells, Methotrexate acts as an allosteric inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in the folic acid metabolic pathway. Folic acid is essential in cells because it is required for thymidine and purine synthesis. Methotrexate therefore acts as a nucleic acid synthesis inhibitor.

    Storage Conditions

    -20 °C

    Tariff Code




    Methotrexate can be used for antitumor studies.

    Cancer Applications

    Methotrexate sodium salt acts as a chemotherapeutic agent by inhibiting nucleic acid synthesis in cancer cells.

    Eukaryotic Cell Culture Applications

    Methotrexate is commonly used in the dihydrofolate reductase (DHFR) selection system as a selection antibiotic to select for DHFR- deficient CHO cells that have been transfected with DHFR genes. Methotrexate inhibits the activity of DHFR; however, cells that overproduce DHFR can tolerate higher concentrations of methotrexate. In most cases, overproducing DHFR cells produce more recombinant protein than lower DHFR producing cells.

    CHO cells and other mammalian cells used in biopharma manufacturing may exhibit toxicity to USP-grade products.  Our Methotrexate, EvoPure® has been purified to remove toxic impurities.  If you have unique specifications for your system, please contact us.

    Insect Biology Applications

    Methotrexate is effective for pyrimethamine-resistant Plasmodium vivax malaria parasites.





    Yellow Powder



    Impurity Profile

    Impurity A| (2,4-Diaminopteridin-6-yl)methanol Hydrochloride|945-24-4|C7H8N6O|192.18| Impurity B| (2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid; N-Desmethyl Methotrexate|54-62-6|C19H20N8O5|440.41| Impurity C| (2S)-2-[[4-[[(2-amino-4-hydroxy pteridin-6-yl) methyl] methyl amino] benzoyl] amino] pentanedioic acid ; Methotrextae 4-Oxo Analog |2410-93-7|C20H21N7O6|455.42| Impurity D| 4-[[(2-amino-4-hydroxypteridin-6-yl)methyl] methylamino]benzoic acid; N10-Methylpteroic acid|5623-18-7|C15H14N6O3|326.31| Impurity E| 4-[[(2,4-Diaminopteridin-6-yl) methyl] methyl amino]benzoic acid; 2,4-Diamino-N10-methylpterioc acid|70844-48-3|C15H15N7O2|325.33| Impurity F| (2R)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ; (R)-Methotrexate||C20H22N8O5|454.44| Methotrexate Dimethyl Ester| N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid dimethyl ester; Dimethyl Methotrexate|34378-65-9|||7-Hydroxy Methotrexate|4-Amino-7-hydroxy-10-methylpteroylglutamic acid|5939-37-7|C20H22N8O6|470.44|



    Fairbanks, LD et al (1999)  Methotrexate inhibits the first committed step of purine biosynthesis in mitogen-stimulated human T-lymphocytes: A metabolic basis for efficacy in rheumatoid arthritis? Biochem. J 342 (1):143-52

    Kingston RE, Kaufman RJ, Bebbington CR and Rolfe MR (2002)  Amplification using CHO cell expression vectors. Curr. Protoc. Mol. Biol. Chapter 16:Unit 16.23  PMID 18265304

    Maleki H et al (2017)  Methotrexate-loaded PLGA nanoparticles: Preparation, characterization and their cytotoxicity effect on human glioblastoma U87MG cells. Med. Nano. Res. 4(1):020

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