• Methotrexate sodium salt packaged and labeled.

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SKU: M031

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Description

Methotrexate sodium is a selective agent for DHFR transfected cells and an anticancer compound that inhibits the metabolism of folic acid.

TOKU-E offers three forms of methotrexate: methotrexate (M027), methotrexate sodium (M031), and methotrexate, Evopure® (M091). In aqueous solution, methotrexate sodium is freely soluble. Methotrexate is practically insoluble in water.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

    CAS Number

    7413-34-5

    Molecular Formula

    C20H20N8Na2O5

    Molecular Weight

    498.40

    Mechanism of Action

    In cancerous cells, methotrexate acts as an allosteric inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in the folic acid metabolic pathway. Folic acid is essential in cells because it is required for thymidine and purine synthesis. Methotrexate therefore acts as a nucleic acid synthesis inhibitor.

    Storage Conditions

    -20 °C

    Tariff Code

    2933.59.2600

Applications

    Cancer Applications

    Methotrexate sodium salt acts as a chemotherapeutic agent by inhibiting nucleic acid synthesis in cancer cells.

    Eukaryotic Cell Culture Applications

    Methotrexate is commonly used in the dihydrofolate reductase (DHFR) selection system as a selection antibiotic to select for dihydrofolate reductase (DHFR) deficient CHO cells (Chinese hamster ovary) that have been transfected with DHFR genes. Methotrexate inhibits the activity of DHFR; however, cells that overproduce DHFR can tolerate higher concentrations of methotrexate. In most cases, overproducing DHFR cells produce more recombinant protein than lower DHFR producing cells.

Specifications

    Form

    Powder

    Appearance

    Yellow Powder

    Source

    Synthetic

    Impurity Profile

    Impurity A| (2,4-Diaminopteridin-6-yl)methanol Hydrochloride|945-24-4|C7H8N6O|192.18| Impurity B| (2S)-2-[[4-[[(2,4-diaminopteridin-6-yl)methyl]amino]benzoyl]amino]pentanedioic acid; N-Desmethyl Methotrexate|54-62-6|C19H20N8O5|440.41| Impurity C| (2S)-2-[[4-[[(2-amino-4-hydroxy pteridin-6-yl) methyl] methyl amino] benzoyl] amino] pentanedioic acid ; Methotrextae 4-Oxo Analog |2410-93-7|C20H21N7O6|455.42| Impurity D| 4-[[(2-amino-4-hydroxypteridin-6-yl)methyl] methylamino]benzoic acid; N10-Methylpteroic acid|5623-18-7|C15H14N6O3|326.31| Impurity E| 4-[[(2,4-Diaminopteridin-6-yl) methyl] methyl amino]benzoic acid; 2,4-Diamino-N10-methylpterioc acid|70844-48-3|C15H15N7O2|325.33| Impurity F| (2R)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ; (R)-Methotrexate||C20H22N8O5|454.44| Methotrexate Dimethyl Ester| N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid dimethyl ester; Dimethyl Methotrexate|34378-65-9|||7-Hydroxy Methotrexate|4-Amino-7-hydroxy-10-methylpteroylglutamic acid|5939-37-7|C20H22N8O6|470.44|

References

    References

    Fairbanks, L. D., and Et Al. "Methotrexate Inhibits the First Committed Step of Purine Biosynthesis in Mitogen-stimulated Human T-lymphocytes: A Metabolic Basis for Efficacy in Rheumatoid Arthritis?" Biochemistry Journal 342.1 (1999): 143-52. www.ncbi.gov. Web. 6 Sept. 2012.

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