• Hygromycin B, 50mg/ml packaged and labeled.

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SKU: H011

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Description

Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B solution is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells.

This product is packaged as a solution at a concentration of 50 mg/mL in PBS buffer.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

For more hygromycin B products, click here.

For more information on hygromycin B, EvoPure®click here.

    CAS Number

    31282-04-9

    Molecular Formula

    C20H37N3O13

    Molecular Weight

    527.52

    Mechanism of Action

    Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.1010

    Spectrum

    Hygromycin B is effective against eukaryotic and prokaryotic cells.

Applications

    Eukaryotic Cell Culture Applications

    Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.

    For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.

    Microbiology Applications

    Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.

Specifications

    Form

    Solution (sterile)

    Appearance

    Clear and yellowish solution

    Source

    Biosynthetic: produced by Streptomyces hygroscopicus.

    Assay

    Hgromycin B - Not less than 900 µg/mg

    Concentration

    50 +/- 10 mg/mL

References

    References

    Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.

    Schindler, D. "Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes." Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.

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