• Guanidine thiocyanate packaged and labeled.

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SKU: G019

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Description

Guanidine thiocyanate, Ultrapure, is a freely soluble chaotropic agent routinely used for DNA/RNA extraction protocols.

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    CAS Number

    593-84-0

    Molecular Formula

    CN3H5 •HSCN

    Molecular Weight

    118.16

    Mechanism of Action

    Extraction of cellular DNA and RNA releases DNases and RNases. Guanidine thiocyanate is used during cell lysis because of its ability to irreversibly inactivate degrading nucleases. In addition to its chaotropic properties, Guanidine thiocyanate has also shown to inactivate enveloped viruses and some non-enveloped viruses.

    Storage Conditions

    2-8 °C

    Tariff Code

    2925.29.9000

Applications

    Eukaryotic Cell Culture Applications

    Guanidine thiocyanate can be used to isolate RNA from both cell culture and tissue form human and animal sources. Authors extracted a protocol for extracting RNA from human placental tissue and from primary and transformed cell lines of human and animal origin, without any ultracentrifugation steps or phenol extractions. Human skin fibroblasts, primary hepatocytes from rats, and Fu5AH rat hepatoma cell line were used and the RNA extracted was intact, with good yield, iRNAse free and not contaminated by DNA. It was suitable for RT-PCR, Northern blotting, and in vitro protein translation (Zolfaghari et al, 1993).

    Microbiology Applications

    Guanidine thiocyanate is routinely used for DNA/RNA extraction protocols to prevent damage by degredative enzymes.

Specifications

    Form

    Powder

    Appearance

    White crystalline powder

    Source

    Synthetic

    Ammonia

    Not more than 0.05%

    Absorbance

    (6M) 280nm : ≤0.5

    pH

    (6M): 4.5-6.0

    Melting Point

    115-122 °C

    Loss on Drying

    ≤0.3%

References

    References

    Chomczynski P (1993)  A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTech. 15(3):532-534, 536-537  PMID 7692896

    Chomzynski P and Sacchi N 2006)  The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on. Nat. Protoc. 1(2):581-585

    Meyer K, Oesch B, Fatzer R, Zurbriggen A and Vandevelde M (1999)  Detection of bovine spongiform encephalopathy-specific PrPSc by treatment with heat and guanidine thiocyanate.  J. Virol. 73(11):9386-9392

    Roberts PL (2007)  Virus inactivation by protein denaturants used in affinity chromatography. J. Intl. Assn. Biol.  Stand. 35(4):343-347

    Zolfaghari R, Chen X and Fisher EA (1993)  Simple method for extracting RNA form cultured cells and tissue with guanidine salts. Clin. Chem. 39(7):1408-1411

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