• Cephalothin sodium, USP packaged and labeled.

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SKU: C026

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Description

Cephalothin sodium is a first generation cephalosporin antibiotic and is freely soluble in aqueous solution (50 mg/mL).

    CAS Number

    58-71-9

    Molecular Formula

    C16H15N2NaO6S2

    Molecular Weight

    418.42

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Cephalothin sodium is a broad spectrum cephalosporin targeting a wide variety of gram positive and gram negative bacteria especially those which cause respiratory and skin infections.

Applications

    Microbiology Applications

    Cephalothin sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Escherichia coli 0.78 µg/mL - >128 µg/mL
    • Staphylococcus aureus 0.25 µg/mL - >23.9 µg/mL
    • For a complete list of cephalothin MIC values, click here.

    Media Supplements

    Cephalothin can be used as a selective agent in several types of isolation media:

    Columbia Blood Agar - Campylobacter selective supplement (Blaser-Wang)

Specifications

    Form

    Powder

    Appearance

    White or almost white crystalline powder

    Source

    Semi-synthetic

    pH

    4.5-7.0 (250 mg/mL)

    Optical Rotation

    +124° to +134°

    Assay

    ≥ 850µg/mg

    Loss on Drying

    ≤ 1.5%

    Impurities

    Individual Impurity: ≤1.0%
    Total Impurities: ≤3.0%

References

    References

    Georgopapadakou, N. H. "Mechanisms of Action of Cephalosporin 3'-quinolone Esters, Carbamates, and Tertiary Amines in Escherichia Coli." American Society for Microbiology 37.3 (1992): 559-65. Antimicrobial Agents and Chemotherapy. Web. 21 Aug. 2012.

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