• Cephalothin sodium, USP packaged and labeled.

click on thumbnail to zoom
SKU: C026

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Cephalothin sodium, USP is a semisynthetic, beta-lactam, first generation cephalosporin antibiotic with bactericidal activity.  It is effective against Gram-positive and Gram-negative bacteria.  Cephalothin sodium is inactivated by cephalosporinase.  The compound is freely soluble in aqueous solution (50 mg/mL).

    CAS Number

    58-71-9

    Molecular Formula

    C16H15N2NaO6S2

    Molecular Weight

    418.42

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Cephalothin sodium is a broad spectrum cephalosporin targeting a wide variety of Gram-positive and Gram-negative bacteria especially those which cause respiratory and skin infections.

Applications

    Eukaryotic Cell Culture Applications

    In vitro cytotoxicity test for estimating the non-ocular irritation dose of ophthalmic solutions was studied using human epidermal keratinocytes in a monolayer. This system can estimate non-ocular irritation dose in advance of the in vivo tests. (Nagami and Maki, 1993).

    Cephalothin resistance in E. coli was found to be mediated by OxyS RNA, a small non-coding RNA which is induced in response to oxidative stress . OxyS modulates gene expression in multiple pathways to develop resistance. In fact, 17 CRP-associated pathway genes are involved. (Cho and Kim, 2018).

    Microbiology Applications

    Cephalothin sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Escherichia coli 0.78 µg/mL - >128 µg/mL
    • Staphylococcus aureus 0.25 µg/mL - >23.9 µg/mL
    • For a complete list of cephalothin MIC values, click here.

    Media Supplements

    Cephalothin can be used as a selective agent in several types of isolation media:

    Columbia Blood Agar - Campylobacter selective supplement (Blaser-Wang)

Specifications

    Form

    Powder

    Appearance

    White or almost white crystalline powder

    Source

    Semi-synthetic

    pH

    4.5-7.0 (250 mg/mL)

    Optical Rotation

    +124° to +134°

    Assay

    ≥ 850µg/mg

    Loss on Drying

    ≤ 1.5%

    Impurities

    Individual Impurity: ≤1.0%
    Total Impurities: ≤3.0%

References

    References

    Cho H and Kim K (2018) Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes. Biochem. Biophys. Res. Comm 506(1):66-72 PMID 30340824

    Georgopapadakou, NH (1992)  Mechanisms of Action of Cephalosporin 3'-quinolone Esters, Carbamates, and Tertiary Amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3): 559-565

    Nagami K, Maki E (1993) In vitro cytotoxicity test for estimating non-ocular irritation dose of ophthalmic solutions. Cell Biol and Toxicol 9(2):107-118 PMID 8242427

Other Items In This Category