• Ceftazidime pentahydrate packaged and labeled.

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SKU: C059

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Description

Ceftazidime pentahydrate is a third-generation cephalosporin antibiotic and is sparingly soluble in aqueous solution (0.396 mg/mL). Acidic and alkali solvents are also commonly used to facilitate dissolution.

    CAS Number

    78439-06-2

    Molecular Formula

    C22H22N6O7S2 •5H2O

    Molecular Weight

    636.65

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases, however, ceftazidime is stable in the presence of β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.3000

    Spectrum

    Ceftazidime pentahydrate targets primarily gram negative bacteria especially Pseudomonas aeruginosa unlike other third generation cephalosporins.

Applications

    Microbiology Applications

    Ceftazidime pentahydrate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Pseudomonas aeruginosa 1 µg/mL – 64 µg/mL
    • Escherichia coli 0.015 µg/mL – 128 µg/mL
    • For a complete list of ceftazidime MIC values, click here.

    Media Supplements

    Ceftazidime can be used as a selective agent in several types of isolation media:

    PALCAM Agar - PALCAM Selective Supplement

    ChromogenicListeria Agar - Chromogenic Listeria Selective Supplement

    ChromogenicListeria Agar - Chromogenic Listeria Differential Supplement

Specifications

    Form

    Powder

    Appearance

    White or almost white crystalline powder

    Source

    Synthetic

    Impurity Profile

    Impurity A| delta-2-ceftazidime; ?(2RS,6R,7R)-7-[[(2Z)-2-(2-aminothiazol-4-yl)-2-[(1-carboxy-1-methylethoxy)imino]acetyl]amino]-8-oxo-3-[(1-pyridinio)methyl]-5-thia-1-azabicyclo[4.2.0]oct-3-ene-2-carboxylate||C22H24N6O7S2|548.60|
    Impurity B| (6R,7R)-7-[[(E)-2-(2-aminothiazol-4-yl)-2-[(1-carboxy-1-methylethoxy)imino]acetyl]amino]-8-oxo-3-[(1-pyridinio)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate||C22H22N6O7S2|546.58|
    Impurity C| (6R,7R)-2-carboxy-8-oxo-3-(pyridiniomethyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-7-aminium dichloride||C13H13N3O3S|291.33|
    Impurity D| (6R,7R)-7-[[(Z)-2-[[2-(1,1-dimethylethoxy)-1,1-dimethyl-2-oxoethoxy]imino]-2-[2-[(triphenylmethyl)amino]thiazol-4-yl]acetyl]amino]-8-oxo-3-(pyridiniomethyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate||||
    Impurity E| (6R,7R)-7-[[(2Z)-2-(2-aminothiazol-4-yl)-2-[[2-(1,1-dimethylethoxy)-1,1-dimethyl-2-oxoethoxy]imino]acetyl]amino]-8-oxo-3-[(1-pyridinio)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate||C26H30N6O7S2|602.69|
    Impurity F| Pyridine|110-86-1|C5H5N|79.10|
    Impurity G| 2-[[[(1Z)-1-(2-aminothiazol-4-yl)-2-[(oxoethyl)amino]-2-oxoethylidene]amino]oxy]-2-methylpropanoic acid||C11H14N4O5S|314.32|
    Impurity H| (6R,7R)-7-[[(2Z)-2-(2-aminothiazol-4-yl)-2-[(2-methoxy-1,1-dimethyl-2-oxoethoxy)imino]acetyl]amino]-8-oxo-3-[(1-pyridinio)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate||C23H24N6O7S2|560.61| Ceftazidime| Delta-3-IsoMer||C22H22N6O7S25H2O|636.65|

    pH

    3.0-4.0

    Assay

    (On Dried Basis): 95.0-102.0%

    Loss on Drying

    13.0-15.0%

    Residue On Ignition

    ≤0.2%

    Heavy Metals

    ≤20ppm

    Impurities

    Limit of Pyridine: ≤0.12%
    Ceftazidime Polymer: ≤0.3%

References

    References

    Georgopapadakou, N. H. "Mechanisms of Action of Cephalosporin 3'-quinolone Esters, Carbamates, and Tertiary Amines in Escherichia Coli." American Society for Microbiology 37.3 (1992): 559-65. Antimicrobial Agents and Chemotherapy. Web. 21 Aug. 2012.

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