• Cefepime hydrochloride packaged and labeled.

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SKU: C009

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Description

Cefepime Hydrochloride is a broad-spectrum, fourth-generation cephalosporin antibiotic.  It is commonly used in antimicrobial susceptibility testing.  The addition of the hydrochloride salt enhances aqueous solubility.   Cefepime has potential for use as an antitumor agent, showing inhibition of human breast cancer cells in vitro when complexed with manganese.

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    CAS Number

    123171-59-5

    Molecular Formula

    C19H25ClN6O5S2 · HCl · H2O

    Molecular Weight

    571.50

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with penicillin binding protein (PBP) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Interestingly, Cefepime is resistant to various β-lactamases encoded by otherwise resistant β-lactam bacteria strains.

    Storage Conditions

    -20°C. Protect from light.

    Tariff Code

    2941.90.5000

    Spectrum

    Cefepime is a broad-spectrum antibiotic targeting a wide variety of naturally antibiotic resistant Gram-positive and Gram-negative bacteria. Some of these naturally resistant bacteria include Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae.

Applications

    Microbiology Applications

    Cefepime Hydrochloride is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:

    • Pseudomonas aeruginosa 32 µg/mL – 256 µg/mL
    • Staphylococcus aureus 2 µg/mL – 16 µg/mL
    • For a more complete list of cefepime MIC values, click here.

    Cefepime was used in a MALDI-TOF MS-based direct-on-target mirodroplet growth assay as part of a screening panel for rapid detection of ESBL, and AmpC β-Lactamases in Enterobacterales.  (Correa-Martinez et al, 2019). 

Specifications

    Form

    Powder

    Appearance

    White or almost white crystalline powder

    Source

    Semi-synthetic

    Water Content (Karl Fischer)

    3.0-6.0%

    Absorbance

    A400≤0.2

    Assay

    (On Dried Basis): 825 – 911 μg/mg

References

    References

    Georgopapadakou NH and Bertasso A (1993)  Mechanisms of action of Cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli.  Antimicrob. Agents. Chemother. 37(3):559-565

    Zhang Z, Bi C, Fan Y, Wang H and Bao Y (2015) Cefepime, a fourth-generation cephalosporin, in complex with manganese, inhibits proteasome activity and induces the apoptosis of human breast cancer cells. Int. J. Molec. Med. 36(4): 1143-1150

    Cefepime from TOKU-E:

    Correa-Martinez C, Idelevich EA, Sparbier K, Kostrzewa M, and Becker K (2019)  Rapid detection of extended-spectrum B-lactamases (ESBL) and AmpC B-Lactamases in Enterobacterales: Development of a screening panel using the MALDI-TOF MS-based direct-on-target microdroplet growth assay. Front. Microbiol. 10:13  PMID 30733710

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