• Bacitracin B2, EvoPure packaged and labeled.
  • Bacitracin B2, EvoPure Molecular Structure

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SKU: B017

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Description

Bacitracin is comprised of numerous antimicrobial peptide fractions. Notable fractions include A, A1, B, B1, B2, C, D, E, F, G, and X. Standard grade bacitracin (B002) is a mixture of these fractions. Bacitracin A is the most active and potent peptide fraction in bacitracin and is very similar to its bacitracin B counterpart.

For more bacitracin products, including fractions, click here.

    CAS Number

    N/A

    Molecular Formula

    C65H101N17O16S

    Molecular Weight

    1408.69 g/mol

    Mechanism of Action

    Bacitracin prevents phosphorylation of bactoprenol; a transport protein which carries peptidoglycan components outside the cell membrane. Without the active phosphorylated bactoprenol, peptidoglycan synthesis cannot be complete and the cell lyses. Two mechanisms of bacitracin resistance are understood. One mechanism relies on a protein called a BcrABC transporter which pumps bacitracin out after it has entered the cell. An alternative mechanism is due to another protein called BacA which provides the active phosphorylated bactoprenol from a different synthetic pathway.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Bacitracin primarily targets the cell wall in members of the gram positive bacteria including Streptococcus pyogenes and Staphylococcus aureus.

Applications

    Application

    Bacitracin B2, EvoPure® can be used to study properties and characteristics of bacitracin B2 separately from other bacitracin compounds found in standard grade bacitracin. Bacitracin B2, EvoPure® can also be used as an analytical standard.

Specifications

    Appearance

    Beige or white powder

    Source

    Bacillus Subtilis var Tracy

    Water Content (Karl Fisher)

    reported

    Assay

    >90% (HPLC)

Technical Data

Spectral Data - Bacitracin B2, EvoPure®


HNMR Spectra


   

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Solvent: D2O   
Instrument: Mercury 300
Frequency: 300 MHz 



FTIR Spectra


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References

    References

    Stone, K. John, and Jack L. Strominger. "Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate." PNAS 68.12 (1971): 3223-227. Biological Laboratories, Harvard University. Web. 21 Aug. 2012.

    Bell, Robert G. "Preparative High-Performance Liquid Chromatographic Separation and Isolation of Bacitracin Components and Their Relationship to Microbiological Activity." Journal of Chromatography 590 (1992): 163-68. Web. 21 Aug. 2012.

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