SKU: B071

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Description

Bacitracin is comprised of numerous antimicrobial peptide fractions. Notable fractions include A, A1, B, B1, B2, C, D, E, F, G, and X. Standard grade bacitracin (B002) is a mixture of these fractions. Bacitracin A is the most active and potent peptide fraction in bacitracin and is very similar to its bacitracin B counterpart.

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    CAS Number

    NA

    Molecular Formula

    C66H103N17O16S

    Molecular Weight

    1422.71

    Mechanism of Action

    Bacitracin prevents phosphorylation of bactoprenol; a transport protein which carries peptidoglycan components outside the cell membrane. Without the active phosphorylated bactoprenol, peptidoglycan synthesis cannot be complete and the cell lyses. Two mechanisms of bacitracin resistance are understood. One mechanism relies on a protein called a BcrABC transporter which pumps bacitracin out after it has entered the cell. An alternative mechanism is due to another protein called BacA which provides the active phosphorylated bactoprenol from a different synthetic pathway.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Bacitracin primarily targets the cell wall in members of the gram positive bacteria including Streptococcus pyogenes and Staphylococcus aureus.

Applications

    Application

    Bacitracin A1, EvoPure® can be used to study properties and characteristics of bacitracin A1 separately from other bacitracin compounds found in standard grade bacitracin. Bacitracin A1, EvoPure® can also be used as an analytical standard.

Specifications

    Assay

    >95% (HPLC)

References

    References

    Stone, K. John, and Jack L. Strominger. "Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate." PNAS 68.12 (1971): 3223-227. Biological Laboratories, Harvard University. Web. 21 Aug. 2012.

    Bell, Robert G. "Preparative High-Performance Liquid Chromatographic Separation and Isolation of Bacitracin Components and Their Relationship to Microbiological Activity." Journal of Chromatography 590 (1992): 163-68. Web. 21 Aug. 2012.

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