• Bacitracin packaged and labeled.

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SKU: B002

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Bacitracin is a mixture of various related polypeptide antibiotic compounds derived from the bacterium, Bacillus subtilis. Bacitracin inhibits cell wall and peptidoglycan synthesis in Gram-positive bacteria.

Bacitracin is comprised of numerous related compounds and impurities including bacitracins A, B1, B2, B3, F, C1, C2, C3, E, H1, H2, H3, I1, I2, and I3. Standard grade bacitracin is a mixture of these fractions. Bacitracin A has been found to have the most antibacterial activity. Bacitracins B1 and B2 together have a similar antibacterial potency (approx. 90% of bacitracin A). Other bacitracin fractions including bacitracin F and bacitracin Xa have not been studied extensively; however, they appear to have negligible antibacterial activity.

For all TOKU-E bacitracin fractions/components and products, click here.

    CAS Number


    Molecular Formula


    Molecular Weight


    Mechanism of Action

    Bacitracin prevents phosphorylation of bactoprenol; a transport protein which carries peptidoglycan components outside the cell membrane. Without the active phosphorylated bactoprenol, peptidoglycan synthesis cannot be complete and the cell lyses. Two mechanisms of bacitracin resistance are understood. One mechanism relies on a protein called a BcrABC transporter which pumps bacitracin out after it has entered the cell. Another mechanism relies on another protein called BacA which provides the active phosphorylated bactoprenol from a different synthetic pathway.

    Storage Conditions

    2-8 °C

    Tariff Code



    Bacitracin inhibits cell wall synthesis in Gram positive bacteria including Streptococcus pyogenes and Staphylococcus aureus. Representative MIC values include:

    • Staphylococcus aureus 2µg/mL - >32 µg/mL
    • Streptococcus agalactiae >16 µg/mL (Group B)
    • For a complete list of bacitracin MIC values, click here.


    Microbiology Applications

    Bacitracin is a useful tool to differentiate between ß-hemolytic, group A Streptococci (Streptococcus pyogenes) and ß-hemolytic Streptocococci of other groups which include Streptococcus agalactiae. Bacitracin can also be used as a selective supplement in chocolate agar to facilitate the isolation of Haemophilus influenzae.





    White to pale buff powder


    Bacillus subtilis and B. licheniformis.

    Impurity Profile

    Content of Bacitracin A: ≥ 40.0%
    Content of Active Bacitracin: ≥ 70.0% of total area
    Limit of early eluting peptides: ≤ 20.0%
    Limit of Bacitracin F: ≤ 6.0%

    Potency (on a dry basis)

    ≥ 65µ/mg



    Loss on Drying




    Stone, K. John, and Jack L. Strominger. "Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate." PNAS 68.12 (1971): 3223-227. Biological Laboratories, Harvard University. Web. 21 Aug. 2012.

    Bell, Robert G. "Preparative High-Performance Liquid Chromatographic Separation and Isolation of Bacitracin Components and Their Relationship to Microbiological Activity." Journal of Chromatography 590 (1992): 163-68. Web. 21 Aug. 2012.

    MJ Mueller, W Brodschelm. "Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid". Proc. Natl. Acad. Sci. USA Vol. 90, pp. 7490-7494, August 1993.

    "Committee for Veterinary Medicinal Products Bacitracin." Ema.europa.eu. The European Agency for the Evaluation of Medicinal Products, June 1998. Web. 18 Jan. 2013.

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