Amikacin Sulfate, USP (1:2)

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Amikacin Sulfate, USP (1:2)

SKU: A003

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Description

Amikacin Sulfate is broad-spectrum aminoglycoside antibiotic derived from its counterpart, Kanamycin A. Amikacin Sulfate (A003) contains an Amikacin:Sulfate ratio of 1:2.

TOKU-E offers three forms of Amikacin:

All forms have similar potencies and are freely soluble in water (50 mg/mL).

CAS Number

39831-55-5

Molecular Formula

C₂₂H₄₃N₅O₁₃ •2H₂SO₄

Molecular Weight

781.76

Mechanism of Action

Amikacin Sulfate binds to the 30S ribosomal subunit (specifically the 16S rRNA and S12 protein) resulting in interference with the translational initiation complex and mRNA misreading, which leads to a faulty or nonexistent protein.

Storage Conditions

2-8°C

Tariff Code

2941.90.5000

Spectrum

Gram-negative and Gram-positive bacteria. Mycobacterium tubercolosis is also susceptible to Amikacin.

Applications

Eukaryotic Cell Culture Applications

in vitrostudies with Amikacin demonstrate lysosomal phospholipidosis, with the compound binding to the phospholipid bilayer.

Microbiology Applications

Amikacin Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use this information to recommend antibiotic treatment options for infected patients. Samples of microbes grown in presence of a 30 µg Amikacin disc with a zone of inhibition of <14 mm in diameter are considered resistant. Intermediate resistance zones of inhibition are typically 15 mm-16 mm in diameter (1). Representative MIC values include:

Pseudomonas aeruginosa 0.25 µg/mL -512 µg/mL
Serratia marcescens ≤0.25 µg/mL － >32 µg/mL
For a complete list of amikacin MIC values, click here.

Specifications

Form

Powder

Appearance

White crystalline powder

Source

Semi-synthetic

pH

2.0 - 4.0

Optical Rotation

+76° to +84°

Assay

(On Dried Basis): 674-786 µg/mg

Loss on Drying

≤13.0%

Residue On Ignition

≤1.0%

Technical Data

References

Christophe T et al (2009) High content screening identifies decaprenyl-phosphoribose 2’ epimerase as a target for intracellular antimycobacterial inhibitors. PLoS Pathog. 5(10): e10000645. PMID 19876393

Davis, BD (1987) Mechanism of Bactericidal Action of Aminoglycosides. Microbiol Rev 51 (3 ): 341-50 PMID 3312985

Dudek M, Romanowska J, Witula T, Trylska J (2014) Interactions of amikacin with the RNA model of the ribosomal A-site: Computational, spectroscopic and calorimetric studies. Biochimie 102:188-202. PMID 24769038

Laurent G, Carlier MB, Rollman B, Van Hoof F, Tulkens P (1982) Mechanism of aminoglycoside-induced lysosomal phospholipidosis: In vitro and in vivo studies with Gentamicin and Amikacin. Biochem. Pharmacol 31(23):3861-70. PMID 7159463

Singh R, Ray P, Das A, Sharma MJ (2009) Role of persisters and small-colony variants in antibiotic resistance of planktonic and biofilm-associated Staphylococcus aureus: An in vitro study. Med. Microbiol 58(8):1067-1073. PMID 1952816