• Amikacin hydrate packaged and labeled.

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SKU: A002

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Description

Amikacin hydrate is broad spectrum aminoglycoside antibiotic derived from its counterpart kanamycin A.

TOKU-E offers three forms of amikacin: amikacin hydrate (A002), amikacin sulfate (1:2) (A003), and amikacin sulfate (1:1.8) (A070). All forms have similar potencies and are freely soluble in aqueous solution (50 mg/mL).

Sabaeifard et al. used amikacin hydrate from TOKU-E to study its cytotoxicity when used in combination with PLGA nanoparticles. Read more here: "Amikacin loaded PLGA nanoparticles against Pseudomonas aeruginosa."

    CAS Number

    37517-28-5

    Molecular Formula

    C22H43N5O13 •H2O

    Molecular Weight

    585.60 (anhydrous basis)

    Mechanism of Action

    Similar to other aminoglycosides, amikacin binds to the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.5000

    Spectrum

    Species susceptible to amikacin include gram negative bacteria such as Pseudomonas aeruginosa and several key enteric species. In addition, certain non-gram negative species including Mycobacterium tuberculosis have also demonstrated susceptibility to amikacin.

Applications

    Microbiology Applications

    Amikacin hydrate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Medical microbiologists use this information to recommend antibiotic treatment options for infected patients. Samples of microbes grown in presence of a 30 µg amikacin disc with a zone of inhibition of <14 mm in diameter are considered resistant. Intermediate resistance zones of inhibition are typically 15 mm-16 mm in diameter (1). Representative MIC values include:

    • Pseudomonas aeruginosa 0.25 µg/mL -512 µg/mL
    • Serratia marcescens ≤0.25 µg/mL - >32 µg/mL
    • For a complete list of Amikacin MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    White powder

    Source

    Semi-synthetic

    Water Content (Karl Fisher)

    ≤8.5%

    pH

    9.5 - 11.5

    Optical Rotation

    +97° to +105°

    Assay

    (On Dried Basis): ≥900µg/mg

    Residue On Ignition

    ≤1.0%

References

    References

    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

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