• Amikacin hydrate packaged and labeled.

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SKU: A002

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Amikacin is broad-spectrum aminoglycoside antibiotic derived from its counterpart, Kanamycin A.  It interferes with bacterial protein synthesis.  It is commonly used in antimicrobial susceptibility testing.

We also offer:

  • Amikacin Sulfate (1:2) (A003)
  • Amikacin Sulfate (1:1.8) (A070)

All forms have similar potencies and are freely soluble in water (50 mg/mL).

    CAS Number


    Molecular Formula


    Molecular Weight

    585.60 (anhydrous basis)

    Mechanism of Action

    Amikacin Sulfate binds to the 30S ribosomal subunit (specifically the 16S rRNA and S12 protein) resulting in interference with the translational initiation complex and mRNA misreading, which leads to a faulty or nonexistent protein.

    Storage Conditions


    Tariff Code



    Gram-negative and Gram-positive bacteria. Mycobacterium tuberculosis is also susceptible to Amikacin.


    Eukaryotic Cell Culture Applications

    Amikacin demonstrates lysosomal phospholipidosis in vitro, with the compound binding to the phospholipid bilayer.

    Microbiology Applications

    Amikacin is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) and rapid antimicrobial testing devices like the Vitek 2 from Biomerieux against gram negative microbial isolates. Medical microbiologists use this information to recommend antibiotic treatment options for infected patients. Samples of microbes grown in presence of a 30 µg Amikacin disc with a zone of inhibition of <14 mm in diameter are considered resistant. Intermediate resistance zones of inhibition are typically 15 mm-16 mm in diameter (1). Representative MIC values include:

    • Pseudomonas aeruginosa 0.25 µg/mL -512 µg/mL
    • Serratia marcescens ≤0.25 µg/mL - >32 µg/mL
    • For a complete list of Amikacin MIC values, click here.





    White powder



    Water Content (Karl Fischer)



    9.5 - 11.5

    Optical Rotation

    +97° to +105°


    (On Dried Basis): ≥900µg/mg

    Residue On Ignition




    Christophe T et al (2009) High content screening identifies decaprenyl-phosphoribose 2’ epimerase as a target for intracellular antimycobacterial inhibitors. PLoS Pathog. 5(10): e10000645. PMID 19876393

    Davis, BD (1987) Mechanism of Bactericidal Action of Aminoglycosides. Microbiol Rev 51 (3 ): 341-50 PMID 3312985

    Dudek M, Romanowska J, Witula T, Trylska J (2014) Interactions of amikacin with the RNA model of the ribosomal A-site: Computational, spectroscopic and calorimetric studies. Biochimie 102:188-202. PMID 24769038

    Laurent G, Carlier MB, Rollman B, Van Hoof F, Tulkens P (1982) Mechanism of aminoglycoside-induced lysosomal phospholipidosis: In vitro and in vivo studies with Gentamicin and Amikacin. Biochem. Pharmacol 31(23):3861-70. PMID 7159463

    Singh R, Ray P, Das A, Sharma MJ (2009) Role of persisters and small-colony variants in antibiotic resistance of planktonic and biofilm-associated Staphylococcus aureus: An in vitro study. Med. Microbiol 58(8):1067-1073. PMID 1952816

    TOKU-E product citation:

    Parastoo S, Ahya A-A, Soudi MR, Gamazo C and Irache JM (2016). Amikacin loaded PLGA nanoparticles against Pseudomonas aeruginosa. E. J. Pharm. Sci 93:392-398 PMID 27575877

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