• Actinomycin D packaged and labeled.

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SKU: A001

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Description

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis against tumor cells.

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    CAS Number

    50-76-0

    Molecular Formula

    C62H86N12O16

    Molecular Weight

    1255.42

    Mechanism of Action

    Actinomycin is a cyclic polypeptide-containing antibiotic that binds to DNA and inhibits RNA polymerase activity. Because Actinomycin can bind DNA duplexes, it can also interfere with DNA replication, although other chemicals such as hydroxyurea are better suited for use in the laboratory as inhibitors of DNA synthesis. Actinomycin D and its fluorescent derivative,7-aminoactinomycin D (7-AAD), are used as stains in microscopy and flow cytometry applications. The affinity of these stains/compounds for GC-rich regions of DNA strands makes them excellent markers for DNA. 7-AAD binds to single stranded DNA; therefore it is a useful tool in determining apoptosis and distinguishing between dead and alive cells.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1050

Applications

    Cancer Applications

    Actinomycin-D is used to treat Wilms' tumor, rhabdomyosarcoma, germ cell tumors, gestational trophoblastic disease, Ewing's sarcoma, testicular cancer, melanoma, choriocarcinoma, neuroblastoma, retinoblastoma, uterine sarcomas, Kaposi's sarcoma, sarcoma botryoides and soft tissue sarcoma.

    Plant Biology Applications

    Actinomycin D is used in plant tissue culture to inhibit plant growth through tissue deformation. It also acts as an RNA-synthesis inhibitor. Exposure of Actinomycin D at concentrations of 12-20 ug/ml for 3-4 hours in pea and soybean plants caused significant tissue deformation.

Specifications

    Form

    Powder

    Appearance

    Red or orange powder

    Source

    Streptomyces Sp.

    Melting Point

    243°C - 248°C

    Assay

    ≥95.0%

    Loss on Drying

    ≤5.0%

References

    References

    DJ Morre, 1965. Plant Physiology. Changes in tissue deformability accompanying actinomycin D inhibition of plant growth and ribonucleic acid synthesis.

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