SKU: R044

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Description
Rucaparib camsylate inhibits DNA repair enzyme poly-ADP ribose polymerase (PARP), thus it belongs to a class known as PARP inhibitors, which have proven to have anti-cancer activity.  

    CAS Number

    1859053-21-6

    Molecular Formula

    C19H18FN3O ∙ C10H16O4S

    Molecular Weight

    555.66

    Mechanism of Action

    PARP inhibitors prevent the synthesis of poly-ADP ribose by impinging on the downstream DNA repair processes,

    Storage Conditions

    -20C

Applications

    Cancer Applications

    Mutations in BRCA1 and BRCA2 (BRCA1/2), components of the homologous recombination DNA repair pathway, are associated with hereditary breast and ovarian cancers. The anti-tumor activity of Rucaparib was studied in human cancer cell lines carrying a mutated BRCA1/2. Nine human cell lines were studied in vitro: Nonmutated BRCA1/2 (MCF7, MDA-MB-231, HCC1937-BRCA1 [breast cancer] and OSEC-2 [ovarian surface epithelial], mutated BRCA1 (MDA-MB-436, and HCC1937 [breast cancer], mutated BRCA2 (CAPAN-1 [ pancreatic cancer]), heterozygous for BRCA2 (OSEC-1 [ovarian cancer], epigenetically silenced BRCA1 (UACC3199 [breast cancer], and CHO cell lines (parental AA8, XRCC3 mutated IRS 1SF). Rucaparib induced double-strand breaks in all cell lines (Drew et al, 2011). The effectiveness of Rucaparib can be enhanced with radiation. In the first study with prostate cancer cell lines PC3, LNCaP, DU145, and VCaP, the compound radiosensitized the cells, allowing a low dose of radiation over a longer period to enhance DNA damage. LNCaPa and VCaP showed a maximum sensitivity to the compound, followed by PC3 and C4-2 cells (Chatterjee et al, 2013). In neuroblastoma cell lines (SK-N-BE(2c), the combination of Rucaparib with radiation reduced the X-radiation dose required to achieve 50 % cell kill. It is likely that the mechanism of radiosensitisation entails the accumulation of unrepaired radiation-induced DNA damage. (Nile et al, 2016) By combining Rucaparib with radiation, it was possible to reduce the X-radiation dose (or 131I-meta-iodobenzylguanidine activity concentration) required to achieve 50 % cell kill in neuroblastoma SK-N-BE(2c) cell lines. It is likely that the mechanism of radiosensitisation entails the accumulation of unrepaired radiation-induced DNA damage. (Nile et al, 2016)

Specifications

    Form

    solid

    Appearance

    Light yellow to off-white solid

    Source

    synthetic

References

    References

    Chatterjee P., et al. (2013) PARP inhibition sensitizes to low dose-rate radiation TMPRSS2-ERG fusion gene-expressing and PTEN-deficient prostate cancer cells. PLoS One 8: e60408 Drew et al (2011) Therapeutic potential of poly(ADP-ribose) polymerase inhibitor AG014699 in human cancers with mutated or methylated BRCA1 or BRCA2. J. Natl. Cancer Inst. 103: 334-346 Nile DL, Rae C, Hyndman IJ, Gaze MN and Mairs RJ (2016) An evaluation in vitro of PARP-1 inhibitors, Rucaparib and Olaparib, as radiosensitisers for the treatment of neuroblastoma. BMC Cancer 16:621

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