SKU: D022

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Description

De-Plasma™ is a potent and safe anti-Mycoplasma reagent which can be used to eliminate Mycoplasma contamination or can be used prophylactically to prevent contamination.  It consists of a proprietary mixture of three antimicrobial compounds with anti-Mycoplasma properties. 

Mycoplasma belong to a unique genus of bacteria that lack cell walls.  Their small size and ubiquitous distribution account for their ability to go undetected in seemingly sterile cell cultures, but ultimately cause alterations in cell characteristics and processes, leading to inaccurate experimental conclusions.

    CAS Number

    N/A

    Molecular Formula

    N/A

    Molecular Weight

    N/A

    Storage Conditions

    2-8 °C

    Tariff Code

    3821.00.0000

Applications

    Eukaryotic Cell Culture Applications

    TOKU-E’s De-Plasma™ was used to routinely test carcinoma cell lines (HNSCC cell line) including SNU1076, FADU, HSC-2 and HSC-4 (Bdarny M et al, 2018). TOKU-E’s De-Plasma was also used to authenticate the human papilloma virus negative cells (Elkabets et al, 2015).

    Microbiology Applications

    DePlasma™ II is a safe, effective anti-Mycoplasma reagent to avoid or eliminate Mycoplasma contamination. Use at 5 μg/ml for primary cells in culture and 2.5 μg/ml for prophylactic use.

Specifications

    Form

    Powder

    Appearance

    Light to beige colored powder

    Source

    Mixture

References

    References

    References for De-Plasma:

    Bdarny M et al (2018)  The AP-1 complex regulates AXL expression and determines sensitivity to P13Ka inhibition in esophagus and head and neck squamous cell carcinoma. doi: https://doi.org/10.1101/415752 

    Elkabets M et al (2015)  AXL mediates resistance to PI3Kalpha inhibition by activating the EGFR/PKC/mTOR axis in head and neck and esophageal squamous cell carcinomas. Cancer Cell. 27(4):533-546

    Other:

    Drexler, HG (2002)  Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnol. 39(2):75-90

    Van Kuppeveld, FJM (1994)  Detection of Mycoplasma contamination in cell cultures by a Mycoplasma group-specific PCR." Appl. Environ. Microbiol. 60(1): 149-152

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