• Puromycin dihydrochloride, Solution packaged and labeled.

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SKU: P025-P026

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Description

Puromycin dihydrochloride (DiHCl) solution is an aminonucleoside antibiotic solution derived from Streptomyces alboniger. Puromycin DiHCl is routinely used as a selective agent in transfection and transformation protocols.

Puromycin DiHCl solution is prepared at 10 mg/mL in 20 mM HEPES buffer.

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    CAS Number

    58-58-2

    Molecular Formula

    C22H29N7O5•2HCl

    Molecular Weight

    544.43 g/mol

    Mechanism of Action

    During translation, puromycin enters the ribosomal “A” site and disrupts peptide transfer. As a result, the ribosome stops and the peptide chain is terminated leading to a nonfunctional protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Puromycin dihydrochloride is active against both prokaryotic and eurkaryotic cells.

Applications
Specifications

    Form

    Solution

    Appearance

    Clear and colorless or light yellow solution

    Source

    Streptomyces alboniger

    pH

    6.2 - 6.8

References

    References

    Azzam, M. E. "Mechanism of Puromycin Action: Fate of Ribosomes after Release of Nascent Protein Chains from Polysomes." PNAS 70.12 (1973): 3866-3869. www.ncbi.gov. Web. 4 Sept. 2012.

    Vara, J. "Cloning and Expression of a Puromycin N-acetyl Transferase Gene from Streptomyces Alboniger in Streptomyces Lividans and Escherichia Coli." Gene 33.2 (1985): 195-206. Www.ncbi.gov. Web. 7 Sept. 2012.

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