• Nystatin packaged and labeled.

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SKU: N010

N010  
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Description

Nystatin is a fungicidal and fungistatic polyene antifungal and growth promoter.  It functions by accumulating cholesterol and thereby sequesters lipid from cell membranes.  It can be used in quantum dot research, since cholesterol depletion could block several lipid raft-dependent endocytic pathways.

    CAS Number

    1400-61-9

    Molecular Formula

    (Nystatin A1) C47H75NO17

    Molecular Weight

    926.09 (Nystatin A1)

    Mechanism of Action

    Nystatin binds ergosterol in the fungal cell membrane forming pores and increasing permeability. The altered permeability is toxic to the fungi leading to growth inhibition or death.

    Storage Conditions

    -20 °C

    Tariff Code

    2941.20.5000

    Spectrum

    Nystatin primarily targets the cell membrane of Candida species especially those which cause vaginal infections.

Applications

    Eukaryotic Cell Culture Applications

    Nystatin is commonly used to prevent contamination in cell culture. 

    Quantum dots can be used to deliver and monitor biomolecules, but the study of the uptake mechanism is just beginning.  Researchers found that Nystatin is a highly selective inhibitor of the lipid raft-dependent pathway and the uptake of SR9 is a lipid raft-dependent process, thus it can be a valuable compound for quantum dot research  (Xu et al, 2010).

     Nystatin can induce interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in TLR2-expressing THP1 cells.

    Microbiology Applications

    Nystatin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against fungal isolates. Medical microbiologists use AST results to recommend treatment options.  Representative effective ranges include:

      • Candida albicans 0.78 µg/mL - 400 µg/mL

    For a representative list of Nystatin MIC values, click here.

    Plant Biology Applications

    Nystatin can be used in plant tissue culture to control contamination.

Specifications

    Form

    Powder

    Appearance

    Yellow Powder

    Source

    Streptomyces noursei and Streptomyces aureus

    pH

    6.0 - 8.0

    Assay

    (Dried Basis): ≥5000 u/mg

    Loss on Drying

    Not more than 5.0%

    Impurities

    Any Impurity: ≤4.0%

References

    References

    Finkelstein A and Holz R (1973)  Aqueous pores created in thin lipid membranes by the polyene antibiotics Nystatin and Amphotericin B. Mem. 2:377-408  PMID 4585230

    Rice LB and Ghannoum MA (1999)  Antifungal agents: Mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin. Microbiol. Rev. 501-17

    Sevtap Arikan (2002) In vitro activity of Nystatin compared with those of liposomal Nystatin, Amphotericin B, and Fluconazole against clinical Candida isolates. J. Clin. Microbiol. 40(4):1406-1412

    Watts JW and King JM 1973)  The use of antibiotics in the culture of non-sterile plant protoplasts. Planta. 113(30:271-277

    Wilson ZA and Power JB (1989)  Elimination of systemic contamination in explant and protoplast cultures of Rubber (Hevea brasiliensis). Plant Ceil Rep.  7::622-662

    Xu Y et al (2010)  Nona-arginine facilitates delivery of quantum dots into cells via multiple pathways. J. Biomed. Biotechnol 2010 ID 948543, 11 pages



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