• Neomycin sulfate, EP packaged and labeled.

click on thumbnail to zoom
SKU: N012

  Click 'Bulk Order' for 10 or more
Price: make selection(s)

Neomycin Sulfate, EP is a broad-spectrum aminoglycoside antibiotic composed of related compounds including Neomycin A (neamine), Neomycin B (framycetin), Neomycin C, and a few minor compounds.  Neomycin B is the most active, followed by C and A. The quantities of these components vary from lot-to-lot.

Neomycin Sulfate, EP conforms to European Pharmacopoeia specifications.

For additional Neomycin products, click here.

Custom Manufacturing and Testing: We are able to prepare custom Neomycin Sulfate suitable for your unique specifications for use in cell culture, or as ancillary material in upstream biopharma manufacturing.  Additionally, we offer additional testing including endotoxin content, arsenic content, cell line testing, spectral analysis, and more. For more information, please contact us.

    CAS Number


    Molecular Formula

    C23H46N6O13 ·3 H2SO4

    Molecular Weight


    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions


    Tariff Code



    Neomycin is a broad spectrum antibiotic, however, it is mostly used against Gram-negative bacteria.


    Eukaryotic Cell Culture Applications

    Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For complete additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).

    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:

      • Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
      • Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL

                  For a representative list of Neomycin MIC values, click here.

    Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringins and inhibit growth of Enterobacteria and Clostridium bifermentans.

    Plant Biology Applications

    Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium-mediated transformation





    White or yellowish powder


    Streptomyces fradiae.

    Elemental Analysis

    Sulphate: 27.0-31.0%

    Impurity Profile

    Impurity A| 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-streptamine (neamine or neomycin A-LP)|||| Impurity B| 3-N-acetyl-2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-streptamine (3-acetylneamine)|||| Impurity C| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-d-streptamine (paromamine or neomycin D)|||| Impurity D| 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-5-O-[3-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-ribofuranosyl]-d-streptamine (neomycin C)|||| Impurity E| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-5-O-[3-O-(2,6-diamino-2,6-dideoxy-l-idopyranosyl)-d-ribofuranosyl]-d-streptamine (paromomycin I or neomycin E)|||| Impurity F| 4-O-(2-amino-2-deoxy-d-glucopyranosyl)-2-deoxy-5-O-[3-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-d-ribofuranosyl]-d-streptamine (paromomycin II or neomycin F)|||| Impurity G| 3-N-acetyl-2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-d-glucopyranosyl)-5-O-[3-O-(2,6-diamino-2,6-dideoxy-l-idopyranosyl)-d-ribofuranosyl]-d-streptamine (neomycin B-LP)||||



    Optical Rotation

    +53.5° to +59.4°


    (Dried Basis): ≥680IU/mg

    Loss on Drying




    TOKU-E Reference:

    Our Neomycin Sulfate was used to study its synergistic effects with blue light irradiation against S. aureus. as a biofilm disruptor in antimicrobial photodynamic therapy in:  Investigating Inhibitory Synergy between Blue Light Irradiation and Antibiotic Treatment of Staphylococcus aureus.(Fox et al, 2013).

    Other References:

    Aragão FJL and Brasileiro ACM (2002) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-10

    Dai et al (2001)  Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol. Breeding 7: 25–33:2001

    Davis BD (1987)  Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev.  51(3):341-50

    Robertson JH (1971)  Antimicrobial activity of Neomycin C against Staphylococcus epidermidis. App. Micro. 22(6):1164-1165

    Tsuji K and Robertson JH (1969)  Comparative study of responses to Neomycins B and C by microbiological and gas-liquid chromatographic assay methods. App. Microbiol.  18(3):396-398

    Yuan L and Wei H (2006) Rapid analysis of native Neomycin components on a portable capillary electrophoresis system with potential gradient detection. Analytic.  Bioanalyt. Chem. 385(8):1575-1579

Other Items In This Category