• L-Methionine sulfoximine, EvoPure<sup>&reg;</sup>

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L-Methionine Sulfoximine, EvoPure®  is an inhibitor of the enzyme glutamine synthetase. It is the sulfoximine derivative of L-methionine in which the amino group as S-stereochemistry.  It is used as a selection agent in mammalian cell lines.   The product has been fully characterized by FTIR, HNMR, HPLC, and mass spectrometry. It is suitable for use as an ancillary material in upstream biomanufacturing processes, for the  production of recombinant proteins, to select for cells that are overexpressing glutamine synthetase by using glutamine-free media  The use of the MSX-glutamine synthase selection system can be used to produce highly stable, highly amplified clones.

Mammalian cells used in manufacturing applications may respond poorly to research grade L-Methionine sulfoximine, Our EvoPure grade is highly purified and the specifications can be tailored to meet the unique specifications of your manufacturing process.  Please contact us for more information.

L-Methionine Sulfoximine, EvoPure® is soluble in water.

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    Mechanism of Action

    L-Methionine Sulfoximine is a specific and irreversible inhibitor of glutamine synthetase which catalyzes the formation of glutamine from the condensation of glutamate and ammonia.


    Eukaryotic Cell Culture Applications

    L-Methionine sulfoximine is commonly used in the glutamine synthetase or GS system as a selection agent to select for resistant NS0 or other mammalian cells that have been transfected with a transgene complex containing glutamine synthetase and the gene of interest.

    Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins such as antibodies, and cell engineering using a CHO cell expression system (Omasa et al, 2010).






    Omasa T, Onitsuka M, and Kim WD (2020)  Cell engineering and cultivation of Chinese hamster ovary (CHO) cells.  Curr. Pharm. Biotechnol. 11(3):233-240

    Wurm, FM (2016)  Production of recombinant protein therapeutics in cultivated mammalian cells." Nat. Nabiotechnol. 22(11):1393-1398  PMD 15529164

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