SKU: K029

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Description

Kanamycin Acid Sulfate, EW, CulturePure® is an aminoglycoside antibiotic often used to select for bacteria which have been successfully transformed with a plasmid conferring resistance to Kanamycin.

Kanamycin Acid Sulfate, EW, CulturePure® is soluble in water.

CulturePure® products highly purified to remove impurities which could be toxic to mammalian and plant cell cultures, for optimized performance during gene selection.

We also offer:

  • Kanamycin Acid sulfate, BP (K004)
  • Kanamycin Acid Sulfate, EP (K029)

    CAS Number

    64013-70-3

    Molecular Formula

    C18H36N4O11 · 2 H2SO4

    Molecular Weight

    680.65

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit, lodging between the 16S rRNA and S12 protein. This interferes with the translational initiation complex causing misreading of the mRNA, and production of a faulty or nonexistent protein.

    Storage Conditions

    2-8 °C

    Spectrum

    Kanamycin is a broad-spectrum antibiotic; however, it is mostly used against aerobic Gram-negative bacteria.

Applications

    Microbiology Applications

    Kanamycin acid sulfate is commonly used as a selective agent to select for resistant mammalian, fungal, or bacterial cells that contain the kanMX marker or other kanamycin resistance genes. Kanamycin acid sulfate is typically used at a concentration of 50 µg/mL

     Media Supplements

    Kanamycin can be used as a selective agent in several types of isolation media:

    Kanamycin Aesculin Azide AgarEnterococci isolation in food

    Perfringens Agar - SFP and TSC selective supplements for the isolation of Clostridium perfringens

    Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

      • µg/mL
      • x   µg/mL
      • For a representative ist of Kanamycin MIC values, click here.


    Kanamycin is one of the most widely used antibiotics yet its biosynthetic pathway remains unclear. Authors reconstructed the entire biosynthetic pathway through the expression of putative biosynthetic genes from S. kanamyceticus in the non-aminoglycoside-producing Streptomyces venezuelae, demonstrating the potential of pathway engineering to direct in vivo production of more robust aminoglycosides (Park et al, 2011).

    Plant Biology Applications

    Kanamycin is often used in the Agrobacterium mediated transformation while using the npt II gene as selection marker.

Specifications

    Source

    Semi-synthetic. Originally derived from Streptomyces kanamyceticus

References

    References

    TOKU-E Product Reference:

    Pryjma, et al. from the University of British Columbia used Kanamycin Sulfate from TOKU-E to select for transformed kanamycin resistant Campylobacter jejuni cells: "FdhTU-Modulated Formate Dehydrogenase Expression and Electron Donor Availability Enhance Recovery of Campylobacter jejuni following Host Cell Infection"

     

    General References:

    Aragão FJL and Brasileiro ACM (2009) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-10

    Davis, BD (1987)  Mechanism of bactericidal action of aminoglycosides.  Microbiol. Rev. 51(3):341-350

    Park JW et al (2011)  Discovery of parallel pathways of Kanamycin biosynthesis allows antibiotic manipulation. Nat. Chem. Biol. 7(11):843-852 PMID 21983602

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