• Isoniazid packaged and labeled.

click on thumbnail to zoom
SKU: I004

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Isoniazid is a bactericidal and bacteriostatic against Mycobacterium tuberculosis, the causal agent of tuberculosis.

    CAS Number

    54-85-3

    Molecular Formula

    C6H7N3O

    Molecular Weight

    137.14

    Mechanism of Action

    Isoniazid itself is not toxic to the bacterial cell, but acts as a prodrug and is activated by the Mycobacterial enzyme KatG, a catalase-peroxidase.  This enzyme prevents the formation of unstable oxygen radicals.  Once activated, isoniazid binds to and inactivates InhA, an enoyl-reductase involved in mycolic acid synthesis.

    The inhibition of cell wall lipid synthesis, depletion of nucleic acid pools and metabolic depression contribute to the potency and selectivity.

    Storage Conditions

    ≤30°C

    Tariff Code

    2933.99.4600

    Spectrum

    Isoniazid targets Mycoplasma species including M. tuberculosis, M. bovis, and M. kansasii.

Applications

    Microbiology Applications

    A missense mutation within the Mycobacterial inhA gene was shown to confer resistance to Isoniazid in M. smegmatis and M. bovis. The wild-type inhA gene also conferred resistance when transferred on a multicopy plasmid vector to M. smegmatis and M. bovis BCG. (Banerjee et al, 1994).

Specifications

    Appearance

    White Crystalline Powder

    Source

    Water: very soluble

    pH

    6.0-8.0

    Melting Point

    170-173°C

    Assay

    (On Dried Basis): 99.0-100.5%

    Loss on Drying

    ≤0.5%

    Sulfated Ash

    ≤0.1%

    Heavy Metals

    ≤10ppm

References

    References

    Banerjee A et al (1994)  InhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. 263(5144):227-230

    Johnsson K (1994)  Studies on the mechanism of action of Isoniazid and Ethionamide in the chemotherapy of tuberculosis. Amer. Chem. Soc. Vol 5009-010

    Timmins GS and Deretic V (2006)  Mechanisms of action of Isoniazid. Molec. Microbiol. 62(5):1220-1227

Other Items In This Category