• Gentamicin sulfate, EP

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SKU: G007

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Gentamicin sulfate, EP is an aminoglycoside antibiotic composed of several different gentamicin components. The main components of gentamicin include members of the gentamicin C complex: gentamicin C1gentamicin C1a, and gentamicin C2 which compose approximately 80% of gentamicin and demonstrate the highest antibacterial activity. Gentamicin A, B, X, and a few others make up the remaining 20% of gentamicin and have lower antibiotic activity than the gentamicin C complex. Gentamicin is slightly soluble in aqueous solution (~12.6 mg/mL).

Gentamicin sulfate, EP conforms to European Pharmacopoeia specifications. 

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    CAS Number


    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Tariff Code



    Gentamicin sulfate, EP is a broad spectrum antibiotic targeting a wide range of Gram positive and Gram negative bacteria. Interestingly, gentamicin has been found to be effective against certain ╬▓-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."


    Eukaryotic Cell Culture Applications

    Gentamicin sulfate can be added to cell culture media to prevent contamination by Gram-positive and/or Gram-negative bacteria for maintaining continuous cell lines or when propagating primary cell lines.

    Microbiology Applications

    Gentamicin sulfate is commonly used as a selective agent to select for cells containing the gentamicin resistance gene, aacj-AaphD or aacC1. Gentamicin sulfate is generall used at a concentration of 10 - 20 µg/mL.


    Media Supplements

    Gentamicin can be used as a selective agent in several types of isolation media:

    Columbia Blood Agar - Gardnerella vaginalis Selective Supplement

    VRE Medium - VRE Selective Supplement

    Burkholderia cepacia Agar Base - Burkholderia cepacia Selective Supplement


    Water Content (Karl Fisher)

    Not more than 15.0%


    3.5 - 5.5


    Not less than 590 IU/mg (dried basis)

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