Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

 
 
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G418 disulfate 50mg/ml packaged and labeled.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
G418 disulfate, EvoPure packaged and labeled.
G418 disulfate, EvoPure® is a high purity (≥99.0%) form of G418 disulfate and is free of impurities present in standard grade G418...
Hygromycin B, 50mg/ml packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely used...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® is a solution containing high purity (>99.0%) hygromycin B...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® solution (100 mg/mL) is a solution containing high purity (>99.0%) hygromycin B...
Irgasan (Triclosan) packaged and labeled.
Irgasan (or triclosan) is a broad spectrum anti-fungal and antibiotic. Irgasan is slightly soluble in water and freely soluble...
Kanamycin acid sulfate packaged and labeled.
Kanamycin is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Kanamycin sulfate, USP packaged and labeled.
Kanamycin, USP is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Methotrexate
Methotrexate is a selective agent for DHFR transfected cells and an anticancer...
Mycophenolic acid packaged and labeled.
Mycophenolic acid is an immunosuppressant drug commonly used to prevent post-transplant organ rejection...
Neomycin with 10 mg/mL packaged and labeled.
Neomycin is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
Neomycin sulfate, EP packaged and labeled.
Neomycin sulfate, EP is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
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