Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

 
 
Page 2 of 5 1 2 3 4 5
Ceftazidime hydrochloride
Ceftazidime pentahydrate is a third generation cephalosporin antibiotic.
Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is a naturally-occurring co-metabolite of chloramphenicol in...
Chloramphenicol palmitate packaged and labeled in glass vial.
Chloramphenicol palmitate is prepared by acylation of chloramphenicol with palmitic...
Chloramphenicol succinate packaged and labeled in glass vial.
Chloramphenicol succinate is prepared by acylation of chloramphenicol with succinic...
Chloramphenicol succinate sodium packaged and labeled in glass vial.
Chloramphenicol succinate sodium is the salt prepared from chloramphenicol succinate using the free...
Chloramphenicol packaged and labeled.
Chloramphenicol is a bacteriostatic antibiotic that inhibits bacterial protein synthesis. TOKU-E offers two forms...
Coumermycin A1
Coumermycin A1 is a member of the aminocoumarin family of antibiotics, with antibacterial activity...
Erythromycin packaged and labeled.
Erythromycin is a macrolide antibiotic with a target spectrum similar to penicillin. TOKU-E offers three forms...
Erythromycin Estolate packaged and labeled.
Erythromycin estolate is a macrolide antibiotic with a target spectrum similar to penicillin. TOKU-E offers three forms of erythromycin: erythromycin (E002), erythromycin...
Erythromycin Ethylsuccinate packaged and labeled.
Erythromycin ethylsuccinate is a macrolide antibiotic with a target spectrum similar to penicillin. TOKU-E offers three forms of erythromycin: erythromycin (E002), erythromycin...
Packaged and labeled G418 disulfate.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
G418 disulfate (low endotoxin)
G418 disulfate (low endotoxin) is a form of G418 disulfate with a strict endotoxin content specification of ≤1 EU/mg...
Page 2 of 5 1 2 3 4 5