Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

 
 
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Puromycin dihydrochloride packaged and labeled.
Puromycin dihydrochloride (DiHCl) is an aminonucleoside antibiotic derived from Streptomyces alboniger. Puromycin...
Streptomycin sulfate packaged and labeled.
Streptomycin sulfate is an aminoglycoside used alone or in combination with penicillin in cell culture to...
Tetracycline, EP packaged and labeled.
Tetracycline is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA...
Ampicillin trihydrate, EP packaged and labeled.

Ampicillin Trihydrate is β-lactam antibiotic commonly used as a selectable marker.

Chloramphenicol succinate sodium packaged and labeled in glass vial.
Chloramphenicol succinate sodium is the salt prepared from chloramphenicol succinate.
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® is a highly pure form of Hygromycin B  (>99.0% pure).   
Kanamycin sulfate, USP packaged and labeled.
Kanamycin, USP is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Tobramycin packaged and labeled.
Tobramycin is an aminoglycoside antibiotic derived from Streptomyces tenebrarius...
Chloramphenicol packaged and labeled.
Chloramphenicol is a broad-spectrum bacteriostatic antimicrobial that inhibits bacterial protein synthesis.
Kanamycin acid sulfate packaged and labeled.
Kanamycin is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Phleomycin
Phleomycin is a complex of copper-containing glycopeptides. This member of the bleomycin...
Neomycin sulfate, EP packaged and labeled.
Neomycin sulfate, EP is a broad-spectrum aminoglycoside antibiotic composed of a number of related neomycin...
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