Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

 
 
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G418 disulfate 50mg/ml packaged and labeled.

G418 Disulfate Solution (50 mg/ml) in Water is an aqueous solution of G418 Disulfate.

Hygromycin B, 50mg/ml packaged and labeled.
Hygromycin B Solution (50 mg/mL in PBS Buffer) is a solution containing Hygromycin B formulated in PBS buffer.
Mycophenolic acid packaged and labeled.
Mycophenolic acid is an immunosuppressant drug commonly used to prevent post-transplant organ rejection...
Neomycin with 10 mg/mL packaged and labeled.
Neomycin is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
Puromycin dihydrochloride, Solution packaged and labeled.
Puromycin dihydrochloride solution is an aminonucleoside antibiotic solution derived from Streptomyces alboniger. Puromycin...
Tetracycline, USP packaged and labeled.
Tetracycline is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA...
Ampicillin sodium packaged and labeled.

Ampicillin Sodium is β-lactam antibiotic commonly used as a selectable marker.

Blasticidin S HCl, 10 mg/mL packaged and labeled.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes.
Chloramphenicol succinate packaged and labeled in glass vial.
Chloramphenicol succinate is prepared by acylation of chloramphenicol with succinic anhydride.
Chloramphenicol, Solution (50 mg/ml) packaged and labeled.
Chloramphenicol, Solution (50 mg/ml), is a ready-made solution of Chloramphenicol dissolved in ethanol at 50 mg/ml.  
Erythromycin packaged and labeled.
Erythromycin is a macrolide antibiotic derived from Saccharopolyspora erythraea (formerly Streptomyces erythraeus).
Neomycin sulfate, USP packaged and labeled.
Neomycin sulfate, USP is a broad-spectrum aminoglycoside antibiotic composed of a number of related...
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