Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Chloramphenicol succinate packaged and labeled in glass vial.
Chloramphenicol succinate is prepared by acylation of chloramphenicol with succinic...
Chloramphenicol, Solution (50 mg/ml) packaged and labeled.
Chloramphenicol is a bacteriostatic antibiotic that inhibits bacterial protein synthesis.TOKU-E offers two forms of chloramphenicol...
Hygromycin B Solution (Low Endotoxin)
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus...
L-Methionine sulfoximine, EvoPure<sup>&reg;</sup>

L-Methionine sulfoximine (MSO) is an inhibitor of the enzyme, glutamine synthetase (GS). L-Methionine sulfoximine, EvoPure®...

Neomycin sulfate, USP packaged and labeled.
Neomycin sulfate, USP is a broad-spectrum aminoglycoside antibiotic composed of a number of related...
Puromycin dihydrochloride packaged and labeled.
Puromycin dihydrochloride (DiHCl) is an aminonucleoside antibiotic derived from Streptomyces alboniger. Puromycin...
Tetracycline, EP packaged and labeled.
Tetracycline is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA...
Hygromycin B, Concentrated packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® is a solution containing high purity (>99.0%) hygromycin B...
Kanamycin sulfate, USP packaged and labeled.
Kanamycin, USP is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Toxoflavin packaged and labeled in glass vial.
Toxoflavin (PKF 118-310) is an azapteridine antibiotic produced by Burkholderia gladioli, that has shown potent anticancer and antibiotic properties.  It is an effective antagonist of Tcf4/b-catenin signaling as well as an inhibitor of multiple anticancer targets, such as Survivin, KDM4A, and SIRT1/2.
Hygromycin B, EvoPure<sup>®</sup> (Low Endotoxin)
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus...
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