Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Neomycin sulfate, USP packaged and labeled.
Neomycin sulfate, USP is a broad spectrum aminoglycoside antibiotic composed of a number of related...
Puromycin dihydrochloride packaged and labeled.
Puromycin dihydrochloride is an aminonucleoside antibiotic derived from Streptomyces alboniger. Puromycin...
Hygromycin B, Concentrated packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® is a solution containing high purity (>99.0%) hygromycin B...
Kanamycin sulfate, USP packaged and labeled.
Kanamycin, USP is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Hygromycin B, EvoPure<sup>®</sup> (low endotoxin)
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus...
Kanamycin acid sulfate packaged and labeled.
Kanamycin is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Neomycin sulfate, EP packaged and labeled.
Neomycin sulfate, EP is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
Kanamycin acid sulfate, EP
Kanamycin is an aminoglycoside antibiotic often used to select for bacteria which have been successfully...
Puromycin packaged and labeled in glass vial.
Puromycin is a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as...
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