Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Chloramphenicol succinate packaged and labeled in glass vial.
Chloramphenicol succinate is prepared by acylation of chloramphenicol with succinic anhydride.
Chloramphenicol, Solution (50 mg/ml) packaged and labeled.
Chloramphenicol, Solution (50 mg/ml), is a ready-made solution of Chloramphenicol dissolved in ethanol at 50 mg/ml.  
Hygromycin B Solution (Low Endotoxin)
Hygromycin B Solution (Low Endotoxin) has an endotoxin content ≤10 EU/mg.
L-Methionine sulfoximine, EvoPure<sup>&reg;</sup>

L-Methionine Sulfoximine, EvoPure®  is an inhibitor of the enzyme glutamine synthetase used as a selection agent in bioprocessing.

Neomycin sulfate, USP packaged and labeled.
Neomycin Sulfate, USP is a broad-spectrum aminoglycoside antibiotic.
Puromycin dihydrochloride packaged and labeled.

Puromycin Dihydrochloride (syn: Puromycin DiHCl) is an aminonucleoside antibiotic with anti-trypanosomal and antineoplastic properties. 

Tetracycline, EP packaged and labeled.
Tetracycline is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA...
Hygromycin B, Concentrated packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® is a highly pure form of Hygromycin B  (>99.0% pure).   
Kanamycin sulfate, USP packaged and labeled.

Kanamycin Sulfate, USP is a broad-spectrum aminoglycoside antibiotic used in gene selection. 

Toxoflavin packaged and labeled in glass vial.
Toxoflavin (PKF 118-310) is an azapteridine antibiotic produced by Burkholderia gladioli, that has shown potent anticancer and antibiotic properties.  It is an effective antagonist of Tcf4/b-catenin signaling as well as an inhibitor of multiple anticancer targets, such as Survivin, KDM4A, and SIRT1/2.
Hygromycin B, EvoPure (Low Endotoxin)
Hygromycin B, EvoPure® (Low Endotoxin) has a strict endotoxin content of ≤10 EU/mg.
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