Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Puromycin aminonucleoside packaged and labeled.
Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is...
Tetracycline hydrochloride packaged and labeled.
Tetracycline HCl is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by...
Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is the major product of chloramphenicol acetyltransferase.
G418 Disulfate (Low Endotoxin)
G418 Disulfate (Low Endotoxin) is a form of G418 Disulfate with low endotoxin content  (≤1 EU/mg).
G418 disulfate 50mg/ml packaged and labeled.

G418 Disulfate Solution (50 mg/ml) in Water is an aqueous solution of G418 Disulfate.

Hygromycin B, 50mg/ml packaged and labeled.
Hygromycin B Solution (50 mg/mL in PBS Buffer) is a solution containing Hygromycin B formulated in PBS buffer.
Methotrexate sodium salt packaged and labeled.

Methotrexate sodium salt is a selective agent for DHFR-transfected cells.

Mycophenolic acid packaged and labeled.
Mycophenolic acid is an immunosuppressant drug commonly used to prevent post-transplant organ rejection...
Neomycin with 10 mg/mL packaged and labeled.
Neomycin solution (10 mg/ml Neomycin in 0.9% NaCl) is a ready-to-use preparation of the broad-spectrum aminoglycoside antibiotic.
Puromycin dihydrochloride, Solution packaged and labeled.
Puromycin dihydrochloride solution is an aminonucleoside antibiotic solution derived from Streptomyces alboniger. Puromycin...
Tetracycline, USP packaged and labeled.
Tetracycline is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA...
Blasticidin S HCl, 10 mg/mL packaged and labeled.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes.
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