Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Puromycin aminonucleoside packaged and labeled.
Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is...
Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is a naturally-occurring co-metabolite of chloramphenicol in...
G418 Disulfate (Low Endotoxin)
G418 disulfate (low endotoxin) is a form of G418 disulfate with a strict endotoxin content specification of ≤1 EU/mg...
G418 disulfate 50mg/ml packaged and labeled.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
Hygromycin B, 50mg/ml packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely used...
Methotrexate sodium salt packaged and labeled.
Methotrexate sodium is a selective agent for DHFR transfected cells and an anticancer...
Mycophenolic acid packaged and labeled.
Mycophenolic acid is an immunosuppressant drug commonly used to prevent post-transplant organ rejection...
Neomycin with 10 mg/mL packaged and labeled.
Neomycin is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
Puromycin dihydrochloride, Solution packaged and labeled.
Puromycin dihydrochloride solution is an aminonucleoside antibiotic solution derived from Streptomyces alboniger. Puromycin...
Blasticidin S HCl, 10 mg/mL packaged and labeled.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes and is routinely used in..
Chloramphenicol succinate packaged and labeled in glass vial.
Chloramphenicol succinate is prepared by acylation of chloramphenicol with succinic...
Chloramphenicol, Solution (50 mg/ml) packaged and labeled.
Chloramphenicol is a bacteriostatic antibiotic that inhibits bacterial protein synthesis.TOKU-E offers two forms of chloramphenicol...
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