Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
Page 2 of 4 1 2 3 4
Puromycin aminonucleoside packaged and labeled.
Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is...
Tetracycline hydrochloride packaged and labeled.
Tetracycline HCl is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by...
Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is a naturally-occurring co-metabolite of chloramphenicol in...
G418 Disulfate (Low Endotoxin)
G418 disulfate (low endotoxin) is a form of G418 disulfate with a strict endotoxin content specification of ≤1 EU/mg...
G418 disulfate 50mg/ml packaged and labeled.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
Hygromycin B, 50mg/ml packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely used...
Methotrexate sodium salt packaged and labeled.
Methotrexate sodium is a selective agent for DHFR transfected cells and an anticancer...
Mycophenolic acid packaged and labeled.
Mycophenolic acid is an immunosuppressant drug commonly used to prevent post-transplant organ rejection...
Neomycin with 10 mg/mL packaged and labeled.
Neomycin is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
Puromycin dihydrochloride, Solution packaged and labeled.
Puromycin dihydrochloride solution is an aminonucleoside antibiotic solution derived from Streptomyces alboniger. Puromycin...
Tetracycline, USP packaged and labeled.
Tetracycline is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by preventing amino-acyl tRNA...
Blasticidin S HCl, 10 mg/mL packaged and labeled.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes.
Page 2 of 4 1 2 3 4