Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is a naturally-occurring co-metabolite of chloramphenicol in...
G418 disulfate (low endotoxin)
G418 disulfate (low endotoxin) is a form of G418 disulfate with a strict endotoxin content specification of ≤1 EU/mg...
G418 disulfate 50mg/ml packaged and labeled.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
Hygromycin B, 50mg/ml packaged and labeled.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely used...
Methotrexate sodium salt packaged and labeled.
Methotrexate sodium is a selective agent for DHFR transfected cells and an anticancer...
Mycophenolic acid packaged and labeled.
Mycophenolic acid is an immunosuppressant drug commonly used to prevent post-transplant organ rejection...
Neomycin with 10 mg/mL packaged and labeled.
Neomycin is a broad spectrum aminoglycoside antibiotic composed of a number of related neomycin...
Puromycin dihydrochloride, Solution packaged and labeled.
Puromycin dihydrochloride solution is an aminonucleoside antibiotic solution derived from Streptomyces alboniger. Puromycin...
Blasticidin S HCl, 10 mg/mL packaged and labeled.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes and is routinely used in..
Chloramphenicol succinate packaged and labeled in glass vial.
Chloramphenicol succinate is prepared by acylation of chloramphenicol with succinic...
Hygromycin B solution (low endotoxin)
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus...
L-Methionine sulfoximine, EvoPure<sup>&reg;</sup>

L-Methionine sulfoximine (MSO) is an inhibitor of the enzyme, glutamine synthetase (GS). L-Methionine sulfoximine, EvoPure®...

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