Gene Selection Antibiotics

Transfection is a process of introducing DNA or other nucleic acids into eukaryotic cells. Foreign DNA can be introduced into eukaryotic cells by chemical-based or physical methods which include electroporation and sonoporation. To ensure DNA has integrated into the cell's genome, a selective marker is co-transfected with the desired gene which usually confers resistance to a selective agent or antibiotic. Selective stress is applied to transfected cells by using a selective agent or selection antibiotic. Only cells that contain the desired gene along with a resistance gene will grow and survive in the presence of a selective agent. Frequently used selection antibiotics include G418, hygromycin b, neomycin, and puromycin.

Which of the following antibiotic resistance genes do your transfected cells contain? 

 
 
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Packaged and labeled G418 disulfate.
G418 disulfate is used routinely as a gene selection agent in cell culture.
Hygromycin B packaged and labeled.
Hygromycin B is an aminoglycoside antibiotic routinely used as a selection agent in transfection experiments.
Blasticidin S HCl packaged and labeled in glass bottle.
Blasticidin S HCl is a nucleoside antibiotic used as a selection agent.
Puromycin packaged and labeled in glass vial.
Puromycin is a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as...
Actinomycin D packaged and labeled.
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis...
Blasticidin S packaged and labeled in glass vial.
Blasticidin S is a nucleoside produced by several species of Streptomyces.
G418 disulfate, EvoPure packaged and labeled.

G418 Disulfate, EvoPure® is a highly pure (≥ 99.0%) version of our G418 Disulfate. 

Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® solution (100 mg/mL in PBS Buffer) is a solution containing highly pure (>99.0%) Hygromycin B.
Methotrexate
Methotrexate is a selective agent for DHFR transfected cells and an anticancer agent.
Methotrexate, EvoPure<sup>&reg;</sup>

Methotrexate, EvoPure® has been highly purified and is suitable for use in upstream bioprocessing.

Mitomycin C packaged and labeled.

Mitomycin C is a natural methylazirinopyrroloindoledione antineoplastic antibiotic isolated from Streptomyces spp that inhibits bacterial DNA synthesis.  

Nourseothricin (Streptothricin) Sulfate
Nourseothricin is an antibiotic in the Streptothricin class of antibiotics.
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