SKU: F017

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Description

Framycetin sulfate (syn: Neomycin B sulfate) is the sulfate salt form of Neomycin, a broad-spectrum aminoglycoside antibiotic derived from Streptomyces fradiae.  Neomycin B is ~ 65% more active than its stereoisomer, Neomycin C, and ~ 90% more active than Neomycin A, the minor component of the Neomycin complex.

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  • Neomycin B Sulfate, EvoPure® (N019)

    CAS Number

    4146-30-9

    Molecular Formula

    C23H48N6O17S

    Molecular Weight

    712.72

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit, specifically binding to the 16S rRNA and S12 protein. This results in inability to read mRNA ultimately producing a faulty or nonexistent protein. It also induces misreading of the mRNA template and causes translational frameshift, leading to premature termination and bacterial cell death.

    Storage Conditions

    -20°C

    Spectrum

    broad-spectrum, against Gram-positive and Gram-negative bacteria. Ineffective against fungi, viruses and most anaerobic bacteria.

Applications

    Eukaryotic Cell Culture Applications

    Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For additional information for your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).

    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:

        • Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
        • Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL

    For a representative list of Neomycin MIC values, click here.

    Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringens and inhibit growth  of Enterobacteria and Clostridium bifermentans.

    Plant Biology Applications

    Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium mediated transformation.

Specifications

    Form

    powder

    Appearance

    white powder

    Source

    Streptomyces fradiae

References

    References

    Aragão FJL and Brasileiro ACM (2009) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol., 14(1):1-10

    Dai S et al (2001) Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol. Breeding 7: 25–33

    Davis BD (1987)  Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev.  51(3):341-50

    Hoeschst MR (2000)  Soframycin, framycetin sulfate. In: Welbanks L (ed): Compendium of pharmaceuticals and specialties, 35th ed. Can. Pharma Assn. Ottawa, Ontario, p.1474

    Robertson JH (1971)  Antimicrobial activity of Neomycin C against Staphylococcus epidermidis.  Appl. Microbiol. 22(6): 1164-1165

    Tsuji K and Robertson JH (1969)  Comparative study of responses to Neomycins B and C by microbiological and gas-liquid chromatographic assay methods. App. Microbiol. 18(3):396-398

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