• 24-Epibrassinolide packaged and labeled in glass bottle.

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24-Epibrassinolide (Epibrassinolide) is member of the brassinosteroid family, a steroid-derived plant growth regulator that is biologically active and involved in metabolic manipulation and protection from environmental stress. It is a natural polyhydroxysteroid with structural similarity to the steroid hormones found in vertebrates.  Epibrassinolides are known for their role in promoting cell division and expansion.  Epibrassinolide was found to interfere with polyamine biosynthetic and catabolic pathways, causing a reduction in polyamine levels and inducing apoptosis in various cancer cells.  24-Epibrassinolide is soluble in organic solvents but is sparingly soluble in aqueous buffers.

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    Mechanism of Action

    Epibrassinolide is able to offer protection from environmental stress by increasing metabolism or removal of toxic substances such as pesticides or heavy metals.

    Epibrassinolide can target polyamine metabolism which is having promise in human cancers. The compound can reduce intracellular polyamine levels which causes apoptotic cell death in tumor models.  It can also influence stress-related molecules.

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    Cancer Applications

    Epibrassinolide was found to be a potential apoptotic inducer in cell death of prostate cancer cell lines (LNCaP (AR+) and DU145(AR-).  It did not affect normal prostate epithelial cell lines (PNT1a). Its mode of action involved polyamines which play a role in abnormal cell proliferation.  The compound was found to reduce intracellular polyamine levels which led to apoptotic cell death (Obaken et al, 2014). 

    Epibrassinolide was found to reduced cell viability and colony formation in colon carcinoma cell lines (HCT 116 and HT-29), influencing stress-related and survival signaling molecules (Coskun et al, 2015). 

    Epibrassinolide induced apoptosis in colon cancer cells through activating endoplasmic reticulum stress moderated by calreticulin, a fine tuning chaperone molecule that plays a role in protein folding, as demonstrated in HCT 116, HT29, DLD‐1, and SW480 colon cancer cells (Ref).  Epibrassinolide did not affect non-malignant cells (Obakan-Yerlikaya et al, 2017).

    Plant Biology Applications

    Epibrassinolide was applied to Brassica juncea L. under stress conditions due to exposure to copper metals   and demonstrated its ability to reduce toxicity and restore healthy growth (Sharma, 2007).

    Exogenous Epibrassinolide was applied to grape berry (Vitis vinifera)  and was found to enhance  ripening, the phenolic content, anthocyanin content, and antioxidant capacity in the grape skins, with 0.4 mg/L being optimal (Xi et al, 2013).

    Heavy metals and overuse of pesticides can impact food quality, and Epibrassinolide shows potential to alleviate heavy metals and pesticide stress in plants.  It increases growth, biomass accumulation and photosynthetic efficiency.  The compound scavenges reactive oxygen species, and induces proline protection that maintains osmotic potential and functions in osmo-protection (Shahzad et al, 2018).





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    Coskun D, Obakan P, Arisan E, Coker-Gurkan A and Palavan-Unsal N (2015)  Epibrassinolide alters P13K/MAPK signaling axis via activating Foxo3a-induced mitochondria-mediated apoptosis in colon cancer cells. Exp. Cell Res. 338(1):10-21

    Obakan P (2014)  Activation of polyamine catabolic enzymes involved in diverse responses against

    Epibrassinolide-induced apoptosis in LNCaP and DU145 prostate cancer cell lines. Amino Acids. 46(3):553-564  PMID 23963538

    Obakan P et al (2017)  Calreticulin is a fine tuning molecule in Epibrassinolide-induced apoptosis through activating endoplasmic reticulum stress in colon cancer cells. Mol. Carcinog. 56(6):1603-1619  PMID 28112451

    Shahzad B et al (2018)  Role of 24-Epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. Ecotoxicol. Environ Saf. 147:935-944  PMID 29029379

    Sharma P and Bhardwaj R (2007) Effects of 24-Epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress. Acta Physiol. Plant 29:259–263

    Xi A et al (2013)  Regulating the secondary metabolism in grape berry using exogenous 24-Epibrassinolide for enhanced phenolics content and antioxidant capacity. Food Chem. 141(3):3056-3065  PMID 23871059

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