• Daptomycin packaged and labeled.

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SKU: D024

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Daptomycin is a unique lipopeptide antibiotic derived from Streptomyces roseosporus that is effective against Gram-positive bacteria.  This holoenzyme is composed of three subunits, encoded by the dptA, dptBC, and dptD genes, each responsible for incorporating particular amino acids into the peptide.  The cloned dpt gene cluster allows study of Daptomycin biosynthesis and discovery of novel lipopetides through pathway engineering.  Daptomycin is soluble in water (100 mg/mL), DMSO (100 mM), methanol (5 mg/mL), and ethanol (50 mM).

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    Mechanism of Action

    Daptomycin peptides interact with anionic phospholipids in the membrane of Gram-positive bacteria. Peptides integrate into the membrane by a calcium-mediated process and interact with membrane phospholipid head groups and acyl chains. Finally, a pore or channel is formed which can kill the cell by membrane disruption/depolarization or cellular uptake of daptomycin leading to disruption of intracellular processes such as synthesis of DNA, RNA, and protein which results in bacterial cell death.

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    Daptomycin targets only Gram-positive organisms including GRE (glycopeptide-resistant Enterococci) and MRSA (methicillin-resistant Staphylococcus aureus)

    • Vancomycin resistant Enterococcus faecalis≤0.015 μg/mL - 4 μg/mL
    • Methicillin resistant Staphylcoccus aureus - 0.25 μg/mL - 2 μg/mL

    For a complete list of daptomycin MIC values, click here.


    Microbiology Applications

    Daptomycin has been used to study the impact of Staphylococcal accessory regulator (sarA) on Daptomycin susceptibility of Staphylococcus aureus. The mutation of sarA did not affect the Daptomycin MIC but resulted in increased susceptibility in vivo in the context of an established biofilm (Weiss et al, 2009)





    Pale Yellow to Light Brown Powder


    Streptomyces roseosporus

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    Baltz RH, Miao V and Wrigley SK (2005) Natural products to drugs: daptomycin and related lipopeptide antibiotics. Nat. Prod. Rep. 22(6):717-741 PMID 16311632

    Miao V et al (2006) Genetic engineering in Streptomyces roseosporus to produce hybrid lipopeptide antibiotics. Chem. Biol. 13(3):269-76 PMID 16638532

    Silverman JA, Perlmutter NG, and Shapiro HM (2003) Correlation of daptomycin bactericidal activity and membrane depolarization in Staphylococcus aureus. Antimicrob. Agents Chemother. 47(8):2538-44 PMID 12878516

    Straus, SK and Hancock REW (2006) Mode of action of the new antibiotic for Gram-positive pathogens Daptomycin: Comparison with cationic antimicrobial peptides and lipopeptides. Biomem. 1758(9):1215-1223

    Weiss EC et al (2009) Impact of sarA on daptomycin susceptibility of Staphylococcus aureus biofilms in vivo. Antimicrobial. Agents. Chemother. 53(10):4096-4102 PMID 19651914

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