SKU: C084

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Cycloheximide is a protein synthesis inhibitor in eukaryotic cells and is commonly used to prevent fungal growth in microbiological selective media. 

TOKU-E offers three forms of cycloheximide:

Cycloheximide solution is sterile filtered and dissolved in DMSO at 100 mg/mL.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

    CAS Number


    Molecular Formula


    Molecular Weight


    Mechanism of Action

    Cycloheximide prevents ribosomal translocation during translation as shown in a yeast model. Effective doses are present at concentrations around 200 µM to inhibit yeast growth.

    Storage Conditions



    Microbiology Applications

    Media Supplements

    Cycoloheximide is routinely used as a selection agent in several types of isolation media:

    Columbia Blood AgarCampylobacter selective supplement (Butzler)

    Dermasel agar - Selective supplement for dermatophyte fungi

    Campylobacter Agar - Campylobacter Selective Supplement (Preston)

    Listeria Selective Agar - Listeria Selective Supplement

    Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement

    Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement

    STAA Agar - STAA Selective Supplement

    Legionella CYE Agar - Legionella GVPC Selective Supplement

    Campylobacter Agar - Campylobacter Selective Supplement (Karmali)

    Bolton Broth - Bolton Broth Selective Supplement

    Plant Biology Applications

    Cycloheximide is a frequently used lab reagent to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 µM have fungicidal effects.





    Clear to light yellow solution.


    100 - 105 mg/mL



    Doyle S.M., Diamond M. and McCabe P.F, 2009, Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. Journal of Experimental Botany, Vol. 61, No. 2, pp. 473–482, 2010

    Schneider-Poetsch T., Ju J., Eyler D.E., Dang Y., Bhat S., Merrick W.C., Green R. and Shen B., 2009, Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature chemical biology, vol 6, march 2010.

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