• Colistin sulfate, USP packaged and labeled.

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SKU: C083

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Description

Colistin sulfate, USP (syn: polymyxin E) is a cationic, cyclic polypeptide antibiotic belonging to the polymyxin family.   It is a natural product discovered in 1949 that is nonribosomally synthesized by Bacillus polymyxa subspecies colistinus Koyama. Colistin is mixture of Colistin A, B, and C.  Colistin targets the bacterial cell membrane, leading to reduced permeability and cell death.  It is effective against Gram-negative bacteria.  It is soluble in water (50 mg/ml).

TOKU-E offers three forms of Colistin:

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    CAS Number

    1264-72-8

    Molecular Formula

    C52H98N16O13 •2.5 H2SO4 (Colistin Sulfate A)

    Molecular Weight

    1155.43 (Colistin Sulfate A)

    Mechanism of Action

    Colistin sulfate displaces essential ions (magnesium and calcium) in the lipopolysaccharide layer of the cell wall leading to local disturbance of the outer membrane, which causes increased permeability and eventually cell lysis. Colistin also has anti-endotoxin activity. The endotoxin of Gram-negative bacteria is the lipid A portion of the lipopolysaccharide molecules, and colistin binds and neutralizes the lipolysaccharide (Falagas et al, 2005).

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.1050

    Spectrum

    Colistin sulfate is used primarily against Gram-negative bacteria and can also be used against Mycobacteria.

Applications

    Microbiology Applications

    Colistin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Colistin has also shown high potency against high-resistant superbug strains.  Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Shigella spp. 64 µg/mL -128 µg/mL
    • Haemophilus influenzae 0.4 µg/mL – 0.8 µg/mL
    • For a complete list of colistin MIC values, click here.

    Media Supplement

    Colistin is routinely used as a selection agent in several types of isolation media:

    Columbia Blood AgarCampylobacter selective supplement (Butzler)

    Columbia Blood Agar - Staphylococcus/Streptococcusselective supplement

    Columbia Blood Agar - Streptococcus Selective Supplement

    Listeria Selective Agar - Listeria Selective Supplement

    Listeria Selective Agar - Modified Listeria Selective Supplement

    Plant Biology Applications

    Plasmodiophora brassicae spores were treated with colistin sulfate and used to inoculate hairy roots of cabbage, Chinese cabbage, turnip, and rape in a useful dual culture system for P. brassicae (Asano et al, 1999).

Specifications

    Form

    Powder

    Appearance

    White or almost white powder

    Source

    Bacillus Polymyxa subsp.

    Elemental Analysis

    As≤2 ppm

    Potency (on a dry basis)

    ≥19,900 u/mg

    Absorbance

    A470 ≤ 0.4

    pH

    4.0-7.0

    Optical Rotation

    -63° to -73° (dried substance)

    Loss on Drying

    ≤7.0%

    Sulfated Ash

    ≤1.0%

    Heavy Metals

    ≤20 ppm

References

    References

    Asano T, Kageyama K and Myakumachi (1999) Surface disinfestation of resting spores of Plasmodiophora brassicae used to infect hairy roots of Brassica spp. Phytopathol. 89(4):314-319 PMID 18944777

    Falagas ME and Kasiakou SK and Saravolatz LD(2005) Colistin: the revival of polymyxins for the management of multidrug-resistant gram-negative bacterial infections. Clin. Infect. Dis. 40(9):1333-1341 PMID 15825037

    Koen N, van Breda SV and Loots DT (2018) Elucidating the antimicrobial mechanisms of oclistin sulfate on Mycobacterium tuberculosis using metabolomics. Tuberculosis 1111:14-19 PMID 30029899

    Komura S, Kurahashi K (1979) Partial purification and properties of L-2,4-diaminobutyric acid activating enzyme from a polymyxin E producing organism. J. Biochem (Tokyo) 86:1013-1021

    Leifert C, Ritchie JY and Waites WM (1991) Contaminants of plant-tissue and cell cultures. World J. Microbiol. Biotechnol. 74):452-469 PMID 24425131

    Mueller MJ, Brodschelm W, Spannagl E and Zenk MH (1993) Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc. Natl. Acad. Sci. USA Vol. 90(16):7490-7494 PMID 11607420

    Wallace, SJ et al (2012)Interaction of colistin and colistin methanesulfonate with liposomes: colloidal aspects and implications for formulation J. Pharma Sci 101 (9):3347-3359 PMID 22623044

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