• Chlortetracycline HCl, EP packaged and labeled.

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SKU: C029

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Description

Chlorotetracycline HCl, EP is tetracycline antibiotic and the first antibiotic of the tetracycline class to be discovered. Chlorotetracycline HCl is soluble at 8.6 mg/mL.

Chlortetracycline HCl, EP meets European Pharmacopoeia (EP) specifications.

    CAS Number

    64-72-2

    Molecular Formula

    C22H23ClN2O8· HCl

    Molecular Weight

    515.34 g/mol

    Mechanism of Action

    The mechanism of chlorotetracycline involves entering a cell and binding to the 30s ribosomal subunit preventing peptide elongation and ultimately inhibiting protein synthesis. Resistance to chlorotetracycline can be a result of inactivation by cell enzymes or pumping the antibiotic out of the cell upon entering.

    Tariff Code

    2941.30.0000

    Spectrum

    Chlorotetracycline is a broad spectrum antibiotic targeting a wide variety of gram positive and gram negative bacterial cells.

Applications

    Microbiology Applications

    Chlorotetracycline HCl is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Micrococcus 17 µg/mL
    • For a complete list of chlorotetracycline MIC values, click here.

    Plant Biology Applications

    Chlortetracycline, in combination with colistin and gentamicin have been successfully used in sweet cherry (Prunus avium L). tissue culture to eliminate Pseudomonas spp. contaminants.

Specifications

    Form

    Powder

    Appearance

    Yellow powder

    Source

    Streptomyces aureofaciens.

    Water Content (Karl Fisher)

    ≤2.0%

    Absorbance

    A460≤0.400

    pH

    2.3-3.3

    Optical Rotation

    -235° to -250°

    Sulfated Ash

    ≤ 0.5%

    Heavy Metals

    ≤50ppm

    Impurities

    4-EPI CTC≤4.0%
    CTC-HCl≥89.5% (dried basis)
    TC-HCl≤8.0% (dried basis)
    TC-HCl+CTC-HCl: 94.5-100.5% (dried basis)

References

    References

    Hierowski, Marian. "Inhibition of Chlortetracycline Protein Synthesis in the E. coli In Vitro System.." PNAS 53.3 (1965): 594-99. www.ncbi.gov. Web. 10 Sept. 2012.

    Leifert C., Ritchie J.Y. and Waites W.M., Contaminants of plant-tissue and cell cultures. World Journal of Microbiology and Biotechnology 7. 452469.

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