Cell Culture

Cell culture applications and products by TOKU-E.

Cell culture is a term that most commonly refers to the process of growing eukayrotic cells in a suitable environment outside of the animal tissue (or plant) in which they were isolated. Two common terms, primary culture and cell lines, are frequently used to classify mammalian cells into two different stages or phases of growth. Primary culture refers to a culture which contains cells that have been taken directly from a specimen to an artificial growth environment. Primary cells become cell lines after they have been subcultured or passaged to a new environment containing a new and fresh growth medium. Cultured cells can be manipulated by introducing foreign DNA into the cells to express and synthesize certain proteins using a technique known as transfection or transduction (virus mediated). Despite the name, cell culture does not typically refer to microbiology cell culture which includes culturing various microbes such as fungi, bacteria, and viruses.

TOKU-E provides a wide range of cell culture products which include selection antibiotics, antimycotics, and Mycoplasma detection and elimination products which can be used in transfection applications and to control cell culture contamination. TOKU-E cell culture products are suitable for use in several cell culture types including mammalian cell culture, insect cell culture, and stem cell culture.

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Methotrexate is a selective agent for DHFR transfected cells and an anticancer...
Methotrexate, EvoPure<sup>&reg;</sup>

Methotrexate is a selective agent for DHFR transfected cells and an anticancer compound that inhibits the metabolism...

Mitomycin C packaged and labeled.
Mitomycin C is a methylazirinopyrroloindoledione antineoplastic antibiotic isolated Streptomyces spp.
Nourseothricin (streptothricin) sulfate
Nourseothricin is an antibiotic in the Streptothricin class of antibiotics.
Puromycin aminonucleoside packaged and labeled.
Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is...
Amphotericin B, solubilized packaged and labeled in glass bottle.
Amphotericin B, solublized is a solubilized form of the polyene antifungal or antimycotic, amphotericin B. Amphotericin B is a...
Anisomycin packaged and labeled.
Anisomycin is a sparingly soluble (2 mg/mL) protein synthesis inhibiting antibiotic produced by Streptomyces griseolus. Anisomycin...
Chloramphenicol acetate packaged and labeled in glass vial.
Chloramphenicol acetate is a naturally-occurring co-metabolite of chloramphenicol in...
Ciprofloxacin hydrochloride packaged and labeled.
Ciprofloxacin HCl is a second generation fluoroquinolone antibiotic. TOKU-E offers two forms of ciprofloxacin...
Cyclosporin B, EvoPure packaged and labeled.
Cyclosporin B, EvoPure® is a dihydroxylated metabolite of cyclosporin A (CsA). Cyclosporin B (M-26)
G418 disulfate (low endotoxin)
G418 disulfate (low endotoxin) is a form of G418 disulfate with a strict endotoxin content specification of ≤1 EU/mg...
G418 disulfate 50mg/ml packaged and labeled.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
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