Cell Culture

Cell culture applications and products by TOKU-E.


Cell culture is a term that most commonly refers to the process of growing eukayrotic cells in a suitable environment outside of the animal tissue (or plant) in which they were isolated. Two common terms, primary culture and cell lines, are frequently used to classify mammalian cells into two different stages or phases of growth. Primary culture refers to a culture which contains cells that have been taken directly from a specimen to an artificial growth environment. Primary cells become cell lines after they have been subcultured or passaged to a new environment containing a new and fresh growth medium. Cultured cells can be manipulated by introducing foreign DNA into the cells to express and synthesize certain proteins using a technique known as transfection or transduction (virus mediated). Despite the name, cell culture does not typically refer to microbiology cell culture which includes culturing various microbes such as fungi, bacteria, and viruses.

TOKU-E provides a wide range of cell culture products which include selection antibiotics, antimycotics, and Mycoplasma detection and elimination products which can be used in transfection applications and to control cell culture contamination. TOKU-E cell culture products are suitable for use in several cell culture types including mammalian cell culture, insect cell culture, and stem cell culture.

 
 
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Cyclosporin A, EvoPure packaged and labeled.
Cyclosporin A, EvoPure® is a neutral, cyclic oligopeptide with immunosuppressive activity.
DePlasma™ packaged and labeled.
De-Plasma™ is a safe, non-toxic treatment for Mycoplasma infection in cell culture.
G418 disulfate, EvoPure packaged and labeled.

G418 Disulfate, EvoPure® is a highly pure (≥ 99.0%) version of our G418 Disulfate. 

Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® solution (100 mg/mL in PBS Buffer) is a solution containing highly pure (>99.0%) Hygromycin B.
Methotrexate
Methotrexate is a selective agent for DHFR transfected cells and an anticancer agent.
Methotrexate, EvoPure<sup>&reg;</sup>

Methotrexate, EvoPure® has been highly purified and is suitable for use in upstream bioprocessing.

Mitomycin C packaged and labeled.
Mitomycin C is a methylazirinopyrroloindoledione antineoplastic antibiotic isolated Streptomyces spp.
Nourseothricin (Streptothricin) Sulfate
Nourseothricin is an antibiotic in the Streptothricin class of antibiotics.
Puromycin aminonucleoside packaged and labeled.
Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is...
Spectinomycin dihydrochloride pentahydrate packaged and labeled.
Spectinomycin dihydrochloride pentahydrate is an aminocyclitol antibiotic that is isolated from Streptomyces spectabilis.  Spectinomycin was...
Spectinomycin sulfate packaged and labeled.
Spectinomycin Sulfate is an aminocyclitol antibiotic that is isolated from Streptomyces spectabilis.  Spectinomycin was discovered in...
Tetracycline hydrochloride packaged and labeled.
Tetracycline HCl is a bacteriostatic polyketide antibiotic. Tetracycline HCl inhibits protein synthesis by...
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