• Ceftriaxone sodium, USP packaged and labeled.

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SKU: C022

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Description

Ceftriaxone sodium, USP is a semisynthetic, broad-spectrum, third-generation cephalosporin antibiotic that can be used in proteomics, the study of penicillin-binding proteins, and biosynthetic pathway investigation. It is freely soluble in aqueous solution (105 mg/mL).

TOKU-E offers two forms of Ceftriaxone:

  • Ceftriaxone Sodium, USP (C022)
  • Ceftriaxone Free Acid (C074)

Ceftriaxone sodium, USP conforms to United States Pharmacopeia specifications.

    CAS Number

    104376-79-6 (trihydrate); 74578-69-1 (anhydrous)

    Molecular Formula

    C18H16N8O7S3 · 2Na · 3.5 H2O

    Molecular Weight

    661.60 g/mol

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Like many cephalosporins, ceftriaxone is resistant to a number of β-lactamases. Ceftriaxone can be used to study PBPs.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Ceftriaxone sodium is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria.

Applications

    Eukaryotic Cell Culture Applications

    During in vitro studies with hippocampal astrocytes, researchers found that Ceftriaxone promoted the nuclear translocation of p65 as well as the activation of Akt. It modulated EAAT2 expression (Excitatory Amino Acid Transporter 2) via PI3K/Akt/NF-κB signaling pathway. It may exert protective effects against early brain injury following subarachnoid hemorrhage (SAH).(Feng et al, 2014).

    Using in vitro models with primary human fetal astrocytes, researchers found Ceftriaxone has a neuroprotective effect and can ameliorate specific neurodegenerative diseases by increasing the update of glutamate, an essential neurotransmitter regulating brain function. It stimulates the expression of one of the major glutamate transporters (Excitatory Amino Acid Transporter or EAAT2) via a signaling pathway called the nuclear factor-kB pathway (Lee et al, 2008).

    Microbiology Applications

    Ceftriaxone sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Streptococcus pneumoniae 0.03 µg/mL - 8 µg/mL
    • Streptococcus pyogenes 0.008 µg/mL – 0.5 µg/mL
    • For a complete list of ceftriaxone MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    Off-white to yellow crystalline powder

    Source

    Synthetic

    Water Content (Karl Fischer)

    8.0-11.0%

    pH

    6.0-8.0

    Assay

    (On Dried Basis): ≥795 µg/mg

    Residual Solvents

    N,N-Dimethylanilline: Not more than 20ppm
    2-Ethylhexanoic acid: Not more than 0.8%

References

    References

    Feng D et al (2014) Ceftriaxone alleviates early brain injury after subarachnoid hemorrhage by increasing excitatory amino acid transporter 2 expression via the P13K/Akt/NF-kB signaling pathway. Neurosci. 268:21-32

    Georgopapadakou, NH (1992) Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3): 559-565

    Lee S et al (2008) Mechanism of ceftriaxone induction of excitatory amino acid transporter-2 expression and glutamate uptake in primary human astrocytes. J. Biol. Chem 283: 13116-13123

    Ruzza P et al (2016) Interactions of GFAP with ceftriaxone and phenytoin: SRCD and molecular docking and dynamic simulation. Biochim. Biophys. Acta. 1860(10):2239-2248 PMID 27133445

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