• Ceftiofur Hydrochloride

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SKU: C228

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Description

Ceftiofur Hydrochloride is the hydrochloride salt of Ceftiofur, a broad-spectrum, third-generation β-lactamase resistant cephalosporin commonly used in veterinary research applications..  It was fist described in 1987.  Its metabolite (desfurolyceftiofur) also has antibiotic activity.  The compound is insoluble in water.

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  • Ceftiofur Sodium (C061)

    CAS Number

    103980-44-5

    Molecular Formula

    C19H17N5O7S• HCl

    Molecular Weight

    560.02

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Like many cephalosporins, ceftiofur is resistant to β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Ceftiofur is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram- negative bacteria including the β -lactamase producing strains.

Applications

    Eukaryotic Cell Culture Applications

    Ceftiofur was tested on equine bone marrow-derived mesenchymal stromal cells and found to have no overall effect on cell viability (Parker et al, 2011).

    The influence of Ceftiofur Sodium was examined in vitro on the development of bovine oocytes/embryos during oocyte maturation, oocyte fertilization and embryo culture.  When oocytes were exposed during oocyte maturation of fertilization, it did not affect embryo development, either at low (10, 50 µg/ml) or high (100, 200 µg/ml) concentrations.  However, if fertilized were exposed to 50 µg/ml, it retarded embryo development.  Therefore, longterm exposure to high doses during post-fertilization adversely affects embryo development (Holyoak et al, 2009).

    Microbiology Applications

    Ceftiofur is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram- negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

      • E. coli 0.12 µg/mL – 0.5 µg/mL
      • Salmonella spp. 0.5 µg/mL – 2 µg/mL
      • For a complete list of Ceftiofur MIC values, click here.
    Ceftiofur is resistant to β-lactamases.  Resistant strains of E. coli have been reported. 

     

References

    References

    Georgopapadakou NH (1992) Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3):559-565 PMID 8384817

    Holyoak GR, Wang S, Liu S, Bunch TJ, Evans RC and Bunch TD (2009)  The effects of ceftiofur sodium (axcel) on vovine oocyte and preimplantation embryonic development assessed by in vitro embryo production techniques. J. Vet. PHarmacol. Ther. 21(2):92-98  PMID 9597645

    Parker RA, Clegg PD and Taylor SE (2011)  The in vitro effects of antibiotics on cell viability and gene expression of equine bone marrow-derived mesenchymal stromal cells. Equine Vet. J. 44(3):355-360

    Salmon SA, Watts JL, Yancey RJ (1996)  In vitro activity of Ceftiofur and its primary metabolite, desfuroylceftiofur, against organisms of veterinary importance. J Vet Diagn Invest. 8(3):332-6.  PMID 8844576

    Yancey RJ et al (1987)  Ceftiofur sodium, a broad-spectrum Cephalosporin: Evaluation in vitro and in vivo in mice. Am. J. Vet. Res. 48(7): 1050-1053  PMID 3631686

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